Type I keratinocyte transglutaminase: expression in human skin and psoriasis.
Type I keratinocyte transglutaminase: expression in human skin and psoriasis.
复制标题
I 型角质形成细胞转谷氨酰胺酶：在人类皮肤和牛皮癣中的表达。
DOI：
10.1111/1523-1747.ep12611394
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Duvic,M
中科院分区：
文献类型：
--
作者：
Schroeder,WT;Thacher,SM;Stewart-Galetka,S;Annarella,M;Chema,D;Siciliano,MJ;Davies,PJ;Tang,HY;Sowa,BA;Duvic,M
在培养的人角质形成细胞和角质层中表达的92kD转谷氨酰胺酶(TGase K)催化了终末分化角化包膜形成的关键步骤。用兔抗TGase K多克隆抗体从人角质形成细胞表达文库中分离重叠克隆。对克隆进行了测序，并与纯化酶中两个溴化氰片段的N端氨基酸序列进行了比较，确定其为TGase K。Tgase K的mRNA在培养的角质形成细胞中表达，但在A431鳞癌细胞、成纤维细胞或其他非上皮性组织和细胞中不表达。虽然TGase K蛋白的表达仅限于正常表皮的上层，但其mRNA普遍存在于整个表皮，这表明转录后调控的可能性。银屑病皮损中存在TGase K蛋白的早熟表达，定量Northern印迹分析表明银屑病皮损中TGase K基因表达水平升高。激光扫描共聚焦显微镜(LSCM)和原位杂交结果显示，银屑病皮损中TGase K基因的表达增加了3~7倍，明显高于正常皮肤和配对相邻皮肤。定量激光扫描共聚焦显微镜为皮肤基因表达分析提供了一种强有力的、直接的方法。
A 92-kD transglutaminase (TGase K), expressed in human cultured keratinocytes and stratum corneum, catalyzes a critical step in the formation of the cornified envelope of terminal differentiation. A rabbit polyclonal antibody to TGase K was used to isolate overlapping cDNA clones from a human keratinocyte cDNA expression library. The cDNA clones were sequenced and unequivocally identified as TGase K by comparison to the N-terminal amino acid sequences of two cyanogen bromide fragments from the purified enzyme. The mRNA for Tgase K is expressed in cultured keratinocytes but not in A431 squamous carcinoma cells, in fibroblasts, or in other non-epithelial tissues and cells. Although TGase K protein expression is limited to the upper layers of normal epidermis, the mRNA is generally present throughout the epidermis, suggesting the possibility of post-transcriptional regulation. Precocious expression of TGase K protein occurs in psoriasis, and quantitative Northern blot analysis of TGase K mRNA from normal and involved epidermal biopsies from psoriasis patients suggests that TGase K mRNA levels are increased in psoriatic lesions. By using quantitative laser scanning confocal microscopy (LSCM) and in situ hybridization, the increase of the TGase K mRNA was in the range of 3-7 times in the psoriatic epidermis and was significantly higher compared with normal skin and with paired adjacent skin. Quantitative LSCM provides a powerful and direct method for analysis of gene expression in skin.