Soluble factors from bone marrow endothelial cells regulate differentiation and proliferation of hematopoietic and endothelial lineages and embryonic stem cells.
Soluble factors from bone marrow endothelial cells regulate differentiation and proliferation of hematopoietic and endothelial lineages and embryonic stem cells.
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发表时间：
2013-08
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通讯作者：
Qi-ru Wang;Q. Yan
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作者：
Qi-ru Wang;Q. Yan
我们已经建立了骨髓内皮细胞系。本综述重点阐述和分析这种骨髓内皮细胞条件培养基（BMEC-CM）对造血和内皮祖细胞以及胚胎干细胞（ESC）分化和增殖的影响。我们将回顾：（1）BMEC-CM促进造血谱系的增殖和分化； (2) BMEC-CM促进内皮细胞谱系的增殖和分化； (3) BMEC-CM诱导造血干细胞/祖细胞分化为内皮祖细胞； (4)BMEC-CM诱导ESC分化为造血细胞和内皮细胞。我们的结论是，BMEC 分泌的可溶性因子能够支持造血和内皮谱系的增殖和分化。此外，这些可溶性因子诱导造血细胞分化为内皮细胞，并诱导ESC分化为内皮细胞和造血细胞。因此，这项工作提供了证据表明造血和内皮谱系的发育存在密切关系。本公开将有益于缺血性和肿瘤疾病的治疗策略，并提高我们对造血谱系和内皮谱系之间关系的理解。
We have established a bone marrow endothelial cell line. This review focuses on the elucidation and analysis of the effects of this bone marrow endothelial cell-conditioned medium (BMEC-CM) on the differentiation and proliferation of hematopoietic and endothelial progenitors as well as embryonic stem cells (ESCs). We will review that (1) BMEC-CM promotes proliferation and differentiation of hematopoietic lineage; (2) BMEC-CM promotes proliferation and differentiation of endothelial lineage; (3) BMEC-CM induces differentiation of hematopoietic stem cells/progenitors into endothelial progenitors; and (4) BMEC-CM induces differentiation of ESCs into hematopoietic cells and endothelial cells. We conclude that the soluble factors secreted by BMECs are able to support the proliferation and differentiation of hematopoietic and endothelium lineages. Moreover, these soluble factors induce hematopoietic cells to differentiate to endothelial cells, and induce ESCs to differentiate towards both endothelial cells and hematopoietic cells. Therefore, this work provides evidence that a close relationship involved in the development of hematopoietic and endothelial lineage. This disclosure will be beneficial for therapy strategy in the treatment of ischemic and tumor diseases, and improve our understanding of the relationship between hematopoietic and endothelial lineages.