Detection of quantitative trait loci influencing recombination using recombinant inbred lines
Detection of quantitative trait loci influencing recombination using recombinant inbred lines
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DOI：
10.1534/genetics.107.076679
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发表时间：
2007-12-01
期刊：
影响因子：
3.3
通讯作者：
Webert, David F.
中科院分区：
文献类型：
--
作者：
Dole, Jefferey;Webert, David F.
高等植物重组变异的遗传基础是多基因的，尽管其在理论和实践上都很重要，但人们对其了解甚少。本文提出了一种检测影响重组自交系（RILs）重组的数量性状基因座（QTL）的方法，该方法依赖于RILs内的基因型数据携带过去重组的特征。行为的分离遗传变异的染色体交叉（重组）的数量在几代自，全同胞，和半同胞产生的RILs没有真正的优势杂交。这种遗传方差，作为总表型方差的一部分，有助于统计的力量的方法，是渐近最大的半同胞，同胞少，最少与自交。检测重组QTL的统计能力随着QTL效应、基因组靶大小和标记密度的减小而下降。相当紧。标记连锁力是更大的较低强度的近亲繁殖后代，反之亦然，为早代。发现了分离方差和统计功效的世代最佳值，其起始值和窄度随标记密度和交配设计而变化，标记连锁越松散越明显。应用该方法对自交系Mo17和B73的RIL群体进行分析，发现了两个影响特定染色体的QTL（LOD > 2.4），并通过典型转化检测到另一个QTL。然而，排列测试未能支持它们的存在（experimentwise alpha = 0.05）。具有更大统计能力并专门选择用于重组QTL分离的其他群体将更有效。
The genetic basis of variation in recombination in higher plants is polygenic and poorly understood, despite its theoretical and practical importance. Here a method of detecting quantitative trait loci (QTL) influencing recombination in recombinant inbred lines (RILs) is proposed that relies upon the fact that genotype data Within RILs carry the signature of past recombination. Behavior of the segregational genetic variance in numbers of chromosomal crossovers (recombination) over generations is described for self, full-sib-, and half-sib-generated RILs with no dominance in true crossovers. This genetic variance, which as a fraction of the total phenotypic variance contributes to the statistical power of the method, was asymptotically greatest with half sibbing, less with sibbing, and least with selfing. The statistical power to detect a recombination QTL declined with diminishing QTL effect, genome target size, and marker density. For reasonably tight. marker linkage power was greater with less intense inbreeding for later generations and vice versa for early generations. Generational optima for segregation variance and statistical power were found, Whose onset, and narrowness varied with marker density and mating design, being more pronounced for looser marker linkage. Application of this method to a maize RIL population derived from inbred lines Mo17 and B73 and developed by selfing suggested two putative QTL (LOD > 2.4) affecting certain chromosomes, and Using a canonical transformation another putative QTL was detected. However, permutation tests failed to support their presence (experimentwise alpha = 0.05). Other populations with more statistical power and chosen specifically for recombination QTL segregation would be more effective.