The Optimization of Short-Term Hepatocyte Preservation Before Transplantation.
The Optimization of Short-Term Hepatocyte Preservation Before Transplantation.
复制标题
DOI：
10.1097/txd.0000000000000687
复制
发表时间：
2017-07
影响因子：
2.3
通讯作者：
Goto M
中科院分区：
文献类型：
--
作者：
Fukuoka K;Inagaki A;Nakamura Y;Matsumura M;Yoshida S;Imura T;Igarashi Y;Miyagi S;Ohashi K;Enosawa S;Kamei T;Unno M;Ohuchi N;Satomi S;Goto M
尚未建立短期肝细胞保存的最佳方法。我们最近开发了一种用于胰岛培养和移植的突出的透氧袋（Tohoku Device [TD]）。在这项研究中，我们调查了TD是否也是有效的肝细胞保存，并试图优化其他条件。在以下条件下保存肝细胞，并观察其结果。首先，研究了TD的有效性。其次，比较了肝细胞培养基（HM）和含或不含胎牛血清（FBS）的器官保存液。第三，作为说明，比较FBS和人血清白蛋白（HSA）。第四，对低温、室温和高温进行了比较。最后，将不同条件下保存的肝细胞移植到无白蛋白大鼠的肾包膜下间隙中，并评估移植面积。TD组保存的肝细胞存活率明显高于非TD组，其活力和功能维持率也明显高于非TD组。无论是否添加胎牛血清，HM组的成活率均显著高于器官保存液组，而存活率和植板效率则与器官保存液组相近。虽然没有FBS的组的存活率极低，但补充HSA的组的结果并不劣于补充FBS的组。高温保存的肝细胞效果最差。TD组植苗面积有高于其他各组的趋势。TD对短期肝细胞保存有效。HSA是FBS的有效替代品，建议在HM中低温保存。
No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte preservation and tried to optimize other conditions. Hepatocytes were preserved in the following conditions, and their outcomes were observed. First, the effectiveness of TD was investigated. Second, hepatocyte medium (HM) and organ preservation solutions with or without fetal bovine serum (FBS) were compared. Third, as supplementations, FBS and human serum albumin (HSA) were compared. Fourth, low, room and high temperature were compared. And finally, hepatocytes preserved in various conditions were transplanted into the subrenal capsule space of nonalbumin rats and engrafted areas were assessed. The survival rate of hepatocytes preserved in TD tended to be higher and their viability and function were maintained significantly greater than those of non-TD group. Irrespective of FBS supplementation, the survival rate of HM group was significantly higher than those of organ preservation solution group while viabilities and plating efficiency were similar among them. Although survival rates of groups without FBS were extremely low, results of HSA supplemented group were not inferior to FBS supplemented group. Hepatocytes preserved at high temperature had the worst results. The engrafted area of TD group tended to be higher than those of other groups. TD is effective for short-term hepatocyte preservation. HSA is a useful substitute for FBS, and preserving in HM at low temperature is recommended.