Changes in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate
Changes in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate
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DOI：
10.1007/s004180050445
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发表时间：
1999-10-01
影响因子：
2.3
通讯作者：
Fujimiya, M
中科院分区：
文献类型：
--
作者：
Oshima, S;Fujimura, M;Fujimiya, M
血清素在炎症性肠道疾病发病机制中的作用尚未得到充分研究。我们研究了葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎的肠粘膜中5-羟色胺水平和含降钙素的肠嗜铬细胞（EC）和肥大细胞密度的变化。大鼠用1.5%DSS处理1个月。血清素水平进行了生化测定，上皮EC细胞和粘膜肥大细胞的密度进行了定量的血清素免疫组化。DSS导致慢性腹泻导致营养不良。在具有完整上皮的结肠壁中显微镜下观察到浸润的炎性细胞。与对照组大鼠相比，DSS治疗组大鼠近端和远端结肠粘膜/粘膜下层组织中的5-羟色胺含量增加。在DSS处理的大鼠的近端和远端结肠中，上皮中EC细胞的密度也增加。与此相反，肥大细胞在固有层的密度显着增加，在远端，但不是在近端结肠DSS治疗的大鼠。这种差异意味着从EC细胞和肥大细胞释放的5-羟色胺可能在DSS诱导的结肠炎的发病机制中发挥不同的作用。
The role of serotonin in the pathogenesis of inflammatory bowel disease has not been fully studied. We examined the changes in the serotonin level and the density of serotonin-containing enterochromaffin (EC) and mast cells in the intestinal mucosa of dextran sodium sulfate (DSS)-induced colitis in rats. Rats were treated with 1.5% DSS for 1 month. Serotonin levels were biochemically measured and the density of epithelial EC cells and mucosal mast cells was quantified by serotonin immunohistochemistry. DSS caused malnutrition due to chronic diarrhea. Infiltrated inflammatory cells were microscopically observed in the colonic wall with intact epithelium. The serotonin content in the mucosa/submucosa tissue was increased in the proximal and distal colon in DSS-treated rats, compared to that in control rats. The density of EC cells in the epithelium also increased in the proximal and distal colon in DSS-treated rats. In contrast, the density of mast cells in the lamina propria dramatically increased in the distal, but not in the proximal colon in DSS-treated rats. This discrepancy implies the serotonin released from EC cells and from mast cells may play different roles in the pathogenesis of DSS-induced colitis.