Fluorescence-Tagged Gold Nanoparticles for Rapidly Characterizing the Size-Dependent Biodistribution in Tumor Models
Fluorescence-Tagged Gold Nanoparticles for Rapidly Characterizing the Size-Dependent Biodistribution in Tumor Models
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DOI：
10.1002/adhm.201200084
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发表时间：
2012-11-01
影响因子：
10
通讯作者：
Chan, Warren C. W.
中科院分区：
文献类型：
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作者：
Chou, Leo Y. T.;Chan, Warren C. W.
纳米颗粒载体可以改善造影剂和治疗剂向患病组织的递送，但是它们的合理设计目前受到缺乏鲁棒技术来实时表征它们的体内行为的阻碍。这项研究表明，荧光标记的金纳米粒子可以优化体内检测，进行纳米粒子设计的药代动力学分析，分析肿瘤外渗，并在荷瘤动物的清除动力学。这种光学成像方法是非侵入性和高通量的。有趣的是，这些荧光金纳米颗粒可用于多光谱成像，以同时比较同一动物体内的几种纳米颗粒设计，并消除测量数据中的宿主依赖性变异性。这些结果共同描述了一种用于评估肿瘤靶向纳米颗粒性能的新平台，并为未来纳米治疗药物的设计提供了新的见解。
Nanoparticle vehicles may improve the delivery of contrast agents and therapeutics to diseased tissues, but their rational design is currently impeded by a lack of robust technologies to characterize their in vivo behavior in realtime. This study demonstrates that fluorescent-labeled gold nanoparticles can be optimized for in vivo detection, perform pharmacokinetic analysis of nanoparticle designs, analyze tumor extravasation, and clearance kinetics in tumor-bearing animals. This optical imaging approach is non-invasive and high-throughput. Interestingly, these fluorescent gold nanoparticles can be used for multispectral imaging to compare several nanoparticle designs simultaneously within the same animal and eliminates the host-dependent variabilities across measured data. Together these results describe a novel platform for evaluating the performance of tumor-targeting nanoparticles, and provide new insights for the design of future nanotherapeutics.