Hydrogen microscopy and analysis of DNA repair using focused high energy ion beams
Hydrogen microscopy and analysis of DNA repair using focused high energy ion beams
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DOI：
10.1016/j.nimb.2006.04.012
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发表时间：
2006-08-01
影响因子：
1.3
通讯作者：
Friedl, A. A.
中科院分区：
文献类型：
--
作者：
Dollinger, G.;Bergmaier, A.;Friedl, A. A.
慕尼黑14 MV串列加速器上的离子微探针SNAKE（Supraleitendes Nanoskop fur Angewandte Kernphysikalische Experimente）通过超导四极偶极子实现束聚焦，并且可以利用宽范围的离子和离子能量，即4-28 MeV质子或高达250 MeV的金离子。由于这些离子束，SNAKE在各个领域的离子束分析中特别有吸引力。这里我们描述SNAKE的两个主要应用。一个是利用高能质子束通过弹性质子-质子散射进行三维氢显微镜的独特可能性。高质子能量允许分析厚度在100 μ m范围内的样品，具有微米分辨率和优于1 ppm的灵敏度。在第二个应用中，SNAKE用于通过用单聚焦离子照射活细胞来分析细胞中的蛋白质动力学。来自免疫染色蛋白53 BP 1的荧光被用作照射HeLa细胞后的生物跟踪检测器。它用于检查照射区域与目标区域的比较。观察到的荧光焦点图案与照射图案相当一致，表明当束斑直径小于0.5 μ m时，总体靶向精度约为2 μ m。这一性能表明SNAKE成功地适应了生物实验，其中细胞通过高能离子在亚细胞水平上靶向。(c)2006 Elsevier B. V.保留所有权利。
The ion microprobe SNAKE (Supraleitendes Nanoskop fur Angewandte Kernphysikalische Experimente) at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, i.e. 4-28 MeV protons or up to 250 MeV gold ions. Due to these ion beams, SNAKE is particularly attractive for ion beam analyses in various fields. Here we describe two main applications of SNAKE. One is the unique possibility to perform three-dimensional hydrogen microscopy by elastic proton-proton scattering utilizing high energy proton beams. The high proton energies allow the analysis of samples with a thickness in the 100 mu m range with micrometer resolution and a sensitivity better than 1 ppm. In a second application, SNAKE is used to analyse protein dynamics in cells by irradiating live cells with single focussed ions. Fluorescence from immunostained protein 53BP1 is used as biological track detector after irradiation of HeLa cells. It is used to examine the irradiated region in comparison with the targeted region. Observed patterns of fluorescence foci agree reasonably well with irradiation patterns, indicating an overall targeting accuracy of about 2 mu m while the beam spot size is less than 0.5 mu m in diameter. This performance shows successful adaptation of SNAKE for biological experiments where cells are targeted on a sub-cellular level by energetic ions. (c) 2006 Elsevier B.V. All rights reserved.