Proteomics of Human Retinal Pigment Epithelium (RPE) Cells.
Proteomics of Human Retinal Pigment Epithelium (RPE) Cells.
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DOI：
10.3390/proteomes6020022
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发表时间：
2018-05-15
期刊：
影响因子：
3.3
通讯作者：
Giorgianni F
中科院分区：
文献类型：
--
作者：
Beranova-Giorgianni S;Giorgianni F
视网膜色素上皮(RPE)是视网膜中一种特殊的多功能细胞，它与感光细胞相邻，形成一层立方的单层极化细胞。RPE是血-视网膜屏障的重要组成部分，对维持视网膜内环境的稳定、支持和感光器的健康起着至关重要的作用。RPE细胞的年龄依赖性、进行性功能障碍和死亡以及由此导致的光感受器的丧失是老年性黄斑变性(AMD)和其他视网膜退行性疾病发生和发展的重要原因。几种不同的RPE细胞培养模型已经被开发和广泛用于RPE的细胞和分子检测，并在培养的RPE的研究中积累了大量关于正常和病理情况下RPE功能的知识。蛋白质组学一直是旨在促进我们对RPE细胞生物学在健康和疾病方面的理解的研究努力的组成部分。本文综述了蛋白质组学在人RPE细胞培养模型的体外定性和定量研究中的应用。讨论的疾病背景集中在AMD。
Retinal pigment epithelium (RPE) are specialized, multifunctional cells in the retina that form a monolayer of cuboidal, polarized cells adjoining the photoreceptor cells. The RPE are a critical component of the blood-retinal barrier, and they play essential functional roles for maintenance of retinal homeostasis and for support and health of photoreceptors. Age-dependent, progressive dysfunction and death of RPE cells and the resultant loss of photoreceptors contribute significantly to the development and progression of age-related macular degeneration (AMD) and other retinal degenerative diseases. Several different RPE cell culture models have been developed and utilized extensively as surrogates for cellular and molecular examinations of the RPE, and a large body of knowledge on RPE function in normal and pathological scenarios has been amassed in studies with cultured RPE. Proteomics has been an integral part of research efforts aimed to advance our understanding of RPE cell biology in health and disease. This review focuses on applications of proteomics to in vitro qualitative and quantitative investigation of human RPE cell culture models. The disease context discussed focuses on AMD.