Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells.
Possible roles of intracellular cyclic AMP, protein kinase C and calcium ion in the apoptotic signaling pathway in bovine luteal cells.
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DOI：
10.1262/jrd.18024
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发表时间：
2006-05
期刊：
影响因子：
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通讯作者：
Yuko Tatsukawa;A. Bowolaksono;R. Nishimura;J. Komiyama;T. Acosta;K. Okuda
中科院分区：
文献类型：
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作者：
Yuko Tatsukawa;A. Bowolaksono;R. Nishimura;J. Komiyama;T. Acosta;K. Okuda
结构性黄体溶解通过黄体细胞凋亡发生。本研究探讨了激活剂的特点，Fas和caspase-3 mRNA的表达和P4的生产在黄体细胞的影响，以跟踪促凋亡和抗凋亡因子在牛黄体（CL）。用环AMP类似物（8-溴环AMP; 8br-cAMP; 2.5 mM）、蛋白激酶C（PKC）激活剂（佛波醇12-肉豆蔻酸酯13-乙酸酯; PMA; 10 μ M）或钙离子载体（A23187; 10 μ M）处理培养的牛黄体中期细胞24 h。8br-cAMP和PMA可抑制Fas和caspase-3 mRNA的表达，而A23187可增加Fas和caspase-3 mRNA的表达（P<0.05）。此外，与对照组相比，8br-cAMP和PMA能促进牛黄体细胞P4的产生，而A23187则抑制其产生（P<0.05）。总体结果表明，cAMP和PKC通过抑制Fas和caspase-3 mRNA的表达和通过刺激P4的产生来抑制牛黄体细胞的凋亡。因此，激活cAMP或PKC的物质可作为牛CL中的存活因子。此外，动员Ca 2+的物质可能通过刺激牛黄体细胞中Fas和caspase-3的表达而作为凋亡因子。
Structural luteolysis occurs by apoptosis of luteal cells. The present study examined the effects of activators of well-characterized second messengers on Fas and caspase-3 mRNA expression and on P4 production in luteal cells in order to trace the pro- and anti-apoptotic factors in the bovine corpus luteum (CL). Cultured bovine mid luteal cells were treated for 24 h with a cyclic AMP analogue (8-bromo cyclic AMP; 8br-cAMP; 2.5 mM), a protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate; PMA; 10 microM), or calcium ionophore (A23187; 10 microM). Fas and caspase-3 mRNA expression was inhibited by 8br-cAMP and PMA but was increased by A23187 (P<0.05). In addition, P4 production by bovine luteal cells was stimulated by 8br-cAMP and PMA, whereas it was inhibited by A23187, compared with untreated controls (P<0.05). The overall results suggest that cAMP and PKC suppress apoptosis in bovine luteal cells through inhibition of Fas and caspase-3 mRNA expression and through stimulation of P4 production. Therefore, substances that activate cAMP or PKC may act as survival factors in the bovine CL. Furthermore, substances that mobilize Ca2+ may act as apoptotic factors by stimulating Fas and caspase-3 expression in the bovine luteal cells.