On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel
On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel
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DOI：
10.3390/jfb11010018
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发表时间：
2020-03
影响因子：
4.8
通讯作者：
Masaru Takeuchi;T. Kozuka;Eunhye Kim;A. Ichikawa;Y. Hasegawa;Qiang Huang;T. Fukuda
中科院分区：
文献类型：
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作者：
Masaru Takeuchi;T. Kozuka;Eunhye Kim;A. Ichikawa;Y. Hasegawa;Qiang Huang;T. Fukuda
我们开发了一种在微流控芯片上使用生物可降解材料制造可移动生物细胞结构的方法。采用光交联可生物降解水凝胶明胶甲基丙烯酸酯（GelMA），在显微镜下利用图案化紫外光制备出任意形状的微结构。在施加疏水涂层材料后，GelMA微结构可在微流体通道内移动。所制造的微结构自组装内的微流控芯片使用我们的方法的流体迫使。通过改变透析温度优化了GelMA的合成工艺，使GelMA保持在适合细胞培养的pH值。将RLC-18大鼠肝细胞（Riken BioResource Research Center，Tsukuba，Japan）在GelMA内部和GelMA微结构上培养以检查细胞生长。然后将细胞在细胞培养物中拉伸1天，并在GelMA微结构上生长良好。然而，它们在GelMA微结构内生长得不好。GelMA微结构在细胞培养4天后部分溶解，因为它们在细胞置于微结构上后具有生物降解性。结果表明，所提出的使用GelMA制造细胞结构的方法可以用作构建模块，在微流体装置内体外组装三维组织样细胞结构。
We developed a procedure for fabricating movable biological cell structures using biodegradable materials on a microfluidic chip. A photo-cross-linkable biodegradable hydrogel gelatin methacrylate (GelMA) was used to fabricate arbitrary microstructure shapes under a microscope using patterned ultraviolet light. The GelMA microstructures were movable inside the microfluidic channel after applying a hydrophobic coating material. The fabricated microstructures were self-assembled inside the microfluidic chip using our method of fluid forcing. The synthesis procedure of GelMA was optimized by changing the dialysis temperature, which kept the GelMA at a suitable pH for cell culture. RLC-18 rat liver cells (Riken BioResource Research Center, Tsukuba, Japan) were cultured inside the GelMA and on the GelMA microstructures to check cell growth. The cells were then stretched for 1 day in the cell culture and grew well on the GelMA microstructures. However, they did not grow well inside the GelMA microstructures. The GelMA microstructures were partially dissolved after 4 days of cell culture because of their biodegradability after the cells were placed on the microstructures. The results indicated that the proposed procedure used to fabricate cell structures using GelMA can be used as a building block to assemble three-dimensional tissue-like cell structures in vitro inside microfluidic devices.