An immunohistochemical method for the detection of proteins in the vertebrate lens
An immunohistochemical method for the detection of proteins in the vertebrate lens
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DOI：
10.1016/s0022-1759(01)00374-x
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发表时间：
2001-07-01
影响因子：
2.2
通讯作者：
Duncan, MK
中科院分区：
文献类型：
--
作者：
Reed, NA;Oh, DJ;Duncan, MK
荧光免疫组织化学传统上难以或不可能在脊椎动物透镜上进行，因为其蛋白质含量极高。这里描述的是一个强大的和快速的方法制备和标记共聚焦显微镜脊椎动物的眼睛。该技术已成功地应用于定位蛋白质在透镜上皮和囊。以及初级和次级纤维。该技术保留了组织形态，并结合了双重和三重标记。允许定位结合到质膜、基底膜、细胞核、内质网以及亚核区室的蛋白质。此外，本技术已被证明可用于包括整个胚胎、成体肌肉、胰腺和肝脏在内的各种组织的荧光免疫组织化学分析。这个程序使我们能够成功地定位各种抗原的不同脊椎动物组织，包括更具挑战性的脊椎动物透镜。(C)2001 Elsevier Science B.V.保留所有权利。
Fluorescence immunohistochemistry has traditionally been difficult or impossible to perform on the vertebrate lens because of its extremely high protein content. Described here is a robust and rapid method for preparing and labeling vertebrate eyes for confocal microscopy. This technique has successfully been applied to localize proteins in the lens epithelium and capsule. as well as the primary and secondary fibers. This technique preserves tissue morphology and coupled with double and triple labeling. has allowed localization of proteins bound to plasma membrane, basement membrane, nucleus, endoplasmic reticulum as well as sub-nuclear compartments. In addition, the present technique has proven useful for fluorescent immunohistochemical analysis of diverse tissues including whole embryos, adult muscle, pancreas, and liver. This procedure allowed us to successfully localize a wide variety of antigens on diverse vertebrate tissues including the more challenging vertebrate lens. (C) 2001 Elsevier Science B.V. All rights reserved.