Increased intake of n-3 polyunsaturated fatty acids elevates the level of apoptosis in the normal sigmoid colon of patients polypectomized for adenomas/tumors
Increased intake of n-3 polyunsaturated fatty acids elevates the level of apoptosis in the normal sigmoid colon of patients polypectomized for adenomas/tumors
复制标题
DOI：
10.1016/s0304383502007176
复制
发表时间：
2003-04-10
期刊：
影响因子：
9.7
通讯作者：
Tokudome, S
中科院分区：
文献类型：
--
作者：
Cheng, JL;Ogawa, K;Tokudome, S
为了阐明n-3多不饱和脂肪酸（PUFAs）对结直肠癌的预防作用，我们对结直肠腺瘤/肿瘤多切除术患者进行了饮食干预。对于前者，给出了以下饮食建议：(1)将脂肪摄入量从总摄入量的30%减少到20%；(2)减少含n-6PUFAs的食物的摄入量，增加n-3 PUFAs的摄入量，持续2年。对照组只建议减少脂肪摄入量（30-20%）。干预期间每年1次结肠镜检查正常乙状结肠粘膜标本，采用免疫染色法检测实验组21例、对照组20例完成干预2年的患者COX-2、细胞增殖（Ki67表达）、053、Bcl-2、Bax，采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸-切口末端标记法（TUNEL）测定凋亡指数（AI）。24个月后，实验组正常乙状结肠黏膜中AI、Bax阳性细胞及Bax/Bcl-2比值显著升高；而在对照组中没有发现这种变化。这些观察结果首次证明了n-3 PUFAs摄入量的增加促进了人类正常结肠黏膜的凋亡，这与对Bax的影响或Bax与Bcl-2的平衡有关。(C) 2003爱思唯尔科学爱尔兰有限公司版权所有。
To clarify preventive effects of n-3 polyunsaturated fatty acids (PUFAs) against colorectal carcinogenesis, we performed a dietary intervention in patients polypectomized for colorectal adenomas/tumors. For the former the following dietary advice was given: (1) decrease intake of fat from 30 to 20% of the total; (2) decrease consumption of n-6PUFAs containing foods, and increase intake of n-3 PUFAs for 2 years. For the comparison group only decreased intake of fat (30-20%) was recommended. Samples of normal sigmoid colon mucosa, obtained by colonoscopic check once a year during the intervention period, were used to investigate COX-2, cell proliferation (Ki67 expression), 053, Bcl-2 and Bax by immunostaining and determine the apoptosis index (AI) by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labeling (TUNEL) in 21 and 20 patients in experimental and comparison groups, respectively, who completed the 2 years of the intervention. After 24 months, the AI and positive cells of Bax and the ratio of Bax/Bcl-2 in normal sigmoid colon mucosa for the experimental group was significantly increased; whereas this change was not found in comparison group. These observations demonstrated for the first time that increased intake of n-3 PUFAs promotes apoptosis of normal colon mucosa in human which is related to effect on Bax or the balance of Bax and Bcl-2. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.