Somatomedin and Thyroid Hormones in the Developing Chick Embryo
Somatomedin and Thyroid Hormones in the Developing Chick Embryo
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发育中的鸡胚中的生长调节素和甲状腺激素
DOI：
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发表时间：
1981
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通讯作者：
R. Wondergem
中科院分区：
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作者：
K. J. Gaspard;H. Klitgaard;R. Wondergem
摘要 胚胎鸡软骨被用来检测生长调节素活性，然而，生长调节素在发育胚胎中的存在尚未见报道。我们已经证明甲状腺激素可以刺激大鼠的生长调节素活性。这项研究比较了发育中的鸡胚中甲状腺激素和生长调节素的变化。测定从孵化第 10 天至第 19 天的胚胎鸡和年轻成年鸡获得的血清中的生长调节素活性、甲状腺素 (T4) 和三碘甲状腺原氨酸 (T3)。生长调节素通过生物测定法测定，该生物测定法测量 12 日龄鸡胚骨盆软骨中硫酸盐的掺入情况。 T4和T3通过放射免疫测定法测定。在 10 至 12 天大的胚胎中未检测到生长调节素活性，在 13 天时可测量到，并在 15 天时增加到峰值（与正常大鼠血清值 1.0 相比，效力为 0.93）。在即将孵化前的血清或幼鸡血清中未检测到生长调节素。 T 直到第 15 天才检测到（0.42 μg T4/100 ml），并在第 19 天增加到 1.39 μg T4/100 ml。在整个研究过程中，T3 存在于血清中，并在第 14 天出现增加（51 ng T3/100 ml）。生长调节素的出现与鸡胚骨骼中胶原蛋白的形成和骨化相一致。正如在大鼠中所证明的那样，甲状腺激素浓度的变化显然不会影响鸡胚中的生长调节素。
Abstract Embryonic chick cartilage is used to detect somatomedin activity, however, the presence of somatomedin in the developing embryo has not been reported. We have shown that thyroid hormones stimulate somatomedin activity in the rat. This study compares changes in thyroid hormones and somatomedin in the developing chick embryo. Somatomedin activity, thyroxine (T4), and triiodothyronine (T3) were determined in sera obtained from embryonic chicks on Days 10 through 19 of incubation and from young adult chickens. Somatomedin was determined by bioassay which measures the sulfate incorporation into pelvic cartilage of 12-day-old chick embryos. T4 and T3 were determined by radioimmunoassay. Somatomedin activity was not detected in 10- to 12-day-old embryos, was measurable at 13 days, and increased to a peak value at 15 days (potency of 0.93 compared to normal rat serum value of 1.0). No somatomedin was detected in the serum immediately prior to hatching or in young chicken serum. T, was not detected until Day 15 (0.42 μg T4/100 ml) and increased to 1.39 μg T4/100 ml on Day 19. T3 was present in the serum throughout the study, with an increase occurring on Day 14 (51 ng T3/100 ml). The appearance of somatomedin coincides with collagen formation and ossification in the chick embryo skeleton. The changes in thyroid hormone concentrations apparently do not influence somatomedin in the chick embryo as has been demonstrated in the rat.