Disease modeling in zebrafish: cancer and immune responses--a report on a workshop held in Spoleto, Italy, July 20-22, 2009.
Disease modeling in zebrafish: cancer and immune responses--a report on a workshop held in Spoleto, Italy, July 20-22, 2009.
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DOI：
10.1089/zeb.2009.0638
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发表时间：
2009-12
期刊：
影响因子：
2
通讯作者：
Trede NS
中科院分区：
文献类型：
--
作者：
Mione M;Meijer AH;Snaar-Jagalska BE;Spaink HP;Trede NS
2007 年在莱顿（荷兰）举办的关于斑马鱼传染病和癌症的斑马鱼研讨会非常成功，强烈促使本报告的作者组织一次后续会议。由于预测微生物和癌细胞的防御机制之间的相似性可以揭示先天免疫反应的特定决定因素的新见解，因此该研讨会重点关注癌症模型和感染研究。由于斑马鱼适合大规模正向和反向遗传筛选，该模型生物非常适合以啮齿动物模型无法比拟的通量水平发现疾病过程中的新基因功能。此外，由于其尺寸小和光学透明，可以在整个生物体水平上研究疾病表现和由此产生的免疫反应。在这方面特别有利的是荧光多色标记技术，它可以标记疾病过程中的参与者（例如癌细胞、免疫细胞和微生物），以便于体内检测。以下报告按时间顺序总结了会议上的口头发言。
The highly successful zebrafish workshop on infectious disease and cancer in zebrafish in Leiden (Netherlands) in 2007 strongly motivated the authors of this report to organize a follow-up meeting.Prompted by the prediction that similarities between defense mechanisms against microbes and cancer cells can reveal new insights into specific determinants of innate immune responses, this workshop focused on cancer models and infection studies. Due to the amenability of zebrafish to large scale forward and reverse genetic screens this model organism is ideal for discovery of novel gene functions in disease processes at a throughput level that can not be matched by rodent models. Furthermore, owing to its small size and optical transparency, disease manifestations and resulting immune responses can be studied at the whole organism level. Particularly advantageous in this context are fluorescence multicolor labeling techniques that allow tagging of the players in disease processes (e.g. cancer cells, immune cells, and microbes) for easy detection in vivo. In the following report the oral presentations at the meeting are summarized chronologically.