Simulated climate warming and mitochondrial haplogroup modulate testicular small non-coding RNA expression in the neotropical pseudoscorpion, Cordylochernes scorpioides
Simulated climate warming and mitochondrial haplogroup modulate testicular small non-coding RNA expression in the neotropical pseudoscorpion, Cordylochernes scorpioides
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DOI：
10.1093/eep/dvy027
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发表时间：
2018-10
影响因子：
3.8
通讯作者：
Eleanor Su-Keene;Melvin M. Bonilla;Michael V. Padua;D. W. Zeh;J. A. Zeh
中科院分区：
文献类型：
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作者：
Eleanor Su-Keene;Melvin M. Bonilla;Michael V. Padua;D. W. Zeh;J. A. Zeh
摘要最近的理论表明，热带陆地节肢动物面临着气候变暖的重大风险。这种离热物种的代谢率随着环境温度的升高呈指数增长，因此在炎热的环境中略有升高的温度可能会比在凉爽的环境中大幅升高的温度产生更大的生理影响。在最近对新热带伪蝎的两项研究中，模拟气候变暖显著降低了存活率、身体大小和性二型性水平。然而，与对男性生育力和女性生育力的灾难性后果相比，这些影响微不足道，这表明生殖是最容易受到气候变暖影响的生命阶段。在这里，我们在线粒体DNA自然发生变异的背景下，研究了慢性高温暴露对蝎子表观遗传调节的影响。表观遗传机制，包括DNA甲基化、组蛋白修饰和小分子非编码RNA(SncRNA)的表达，对温度等环境因素特别敏感，这些因素可以引起表观遗传状态和表型的变化，这些变化可能是世代可遗传的。我们的结果表明，雄性伪蝎暴露在高温下显著改变了睾丸组织中>；60SncRNAs的表达，特别是microRNAs和piwi相互作用的RNAs。线粒体单倍群也是影响SncRNAs和线粒体基因表达的重要因素。这些发现表明，慢性热应激导致表观遗传学特征的改变，这可能是雄性蝎子生殖功能障碍的原因。此外，通过其对表观遗传调控的影响，线粒体DNA多态可能提供对气候变暖的适应性进化反应的可能性。
Abstract Recent theory suggests that tropical terrestrial arthropods are at significant risk from climate warming. Metabolic rate in such ectothermic species increases exponentially with environmental temperature, and a small temperature increase in a hot environment can therefore have a greater physiological impact than a large temperature increase in a cool environment. In two recent studies of the neotropical pseudoscorpion, Cordylochernes scorpioides, simulated climate warming significantly decreased survival, body size and level of sexual dimorphism. However, these effects were minor compared with catastrophic consequences for male fertility and female fecundity, identifying reproduction as the life stage most vulnerable to climate warming. Here, we examine the effects of chronic high-temperature exposure on epigenetic regulation in C. scorpioides in the context of naturally occurring variation in mitochondrial DNA. Epigenetic mechanisms, including DNA methylation, histone modifications and small non-coding RNA (sncRNA) expression, are particularly sensitive to environmental factors such as temperature, which can induce changes in epigenetic states and phenotypes that may be heritable across generations. Our results indicate that exposure of male pseudoscorpions to elevated temperature significantly altered the expression of >60 sncRNAs in testicular tissue, specifically microRNAs and piwi-interacting RNAs. Mitochondrial haplogroup was also a significant factor influencing both sncRNAs and mitochondrial gene expression. These findings demonstrate that chronic heat stress causes changes in epigenetic profiles that may account for reproductive dysfunction in C. scorpioides males. Moreover, through its effects on epigenetic regulation, mitochondrial DNA polymorphism may provide the potential for an adaptive evolutionary response to climate warming.