Epstein-Barr viral DNA: infectivity for human placental cells.
Epstein-Barr viral DNA: infectivity for human placental cells.
复制标题
Epstein-Barr 病毒 DNA：对人类胎盘细胞的感染性。
DOI：
10.1126/science.6259735
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Smith,D
中科院分区：
文献类型：
--
作者：
Miller,G;Grogan,E;Heston,L;Robinson,J;Smith,D
EB病毒（EBV）的纯化DNA通过转染的“钙”方法是有规律地感染的。培养的人胎盘细胞暴露于两种转化株FF 41和B 95的EBV DNA，产生能够将正常B淋巴细胞转化为已建立的细胞系的病毒。在用EBV（FF 41）DNA和EBV（HR-1）DNA处理后，胎盘细胞展示与生产性病毒周期相关的抗原。胎盘细胞没有出现病灶或其他形态学改变的迹象。
Purified DNA of Epstein-Barr virus (EBV) is regularly infectious by means of the "calcium" method of transfection. Cultured human placental cells exposed to EBV DNA of two transforming strains, FF41 and B95, produce virus that is capable of converting normal B lymphocytes into established cell lines. After treatment with EBV (FF41) DNA and EBV (HR-1) DNA the placental cells display antigens associated with the productive viral cycle. The placental cells have not developed foci or other signs of morphologic transformation.