Guided mode resonance sensor for the parallel detection of multiple protein biomarkers in human urine with high sensitivity
Guided mode resonance sensor for the parallel detection of multiple protein biomarkers in human urine with high sensitivity
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DOI：
10.1016/j.bios.2020.112047
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发表时间：
2020-04-01
影响因子：
12.6
通讯作者：
Krauss, Thomas F.
中科院分区：
文献类型：
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作者：
Kenaan, Ahmad;Li, Kezheng;Krauss, Thomas F.
全球医疗保健服务成本的上升和疾病管理的新方法正在推动低成本生物传感模式的发展，例如基于介电共振的无标签光子方法。在这里，我们利用引导模式共振（GMR）传感器的组合传感和成像能力，在未稀释的尿液样本中平行检测多种生物标志物（肌钙蛋白、降钙素原和c反应蛋白）。这种生物传感器的一个关键要求是使用合适的抗体简单而直接地功能化，以确保对疾病特异性的蛋白质生物标志物进行检测。在这里，抗体使用琥珀酰亚胺基[（n -马来酰亚胺丙基）-六乙基乙二醇]酯(SM（PEG)(6)）间隔物固定。与（3-氨基丙基）三乙氧基硅烷（APTES）或右旋糖酐等更常用的方法相比，聚乙二醇（PEG）化学可使所有蛋白质生物标志物的检测限低至10 pg mL（-1）或更高，同时最大限度地减少非特异性结合。我们的方法支持一个简单但高度敏感的诊断平台的愿景，该平台可用于在护理点对各种疾病的患者进行预筛查，从而减轻过度紧张的医疗保健服务的压力。
The rising cost of global healthcare provision and new approaches to managing disease are driving the development of low-cost biosensing modalities, such as label-free photonic methods based on dielectric resonances. Here, we use the combined sensing and imaging capability of a guided mode resonance (GMR) sensor to detect multiple biomarkers (troponin, procalcitonin and C-Reactive Protein) in parallel in undiluted urine samples. A key requirement of such a biosensor is the simple and direct functionalization with suitable antibodies to ensure the disease-specific detection of protein biomarkers. Here, antibodies were immobilized using a succinimidyl[(N-maleimidopropionamido)-hexaethyleneglycol] ester (SM(PEG)(6)) spacer. The polyethylene glycol (PEG) chemistry enables low detection limits of 10 pg mL(-1) or better for all protein biomarkers, while minimizing non-specific binding compared to more commonly used strategies such as (3-Aminopropyl)triethoxysilane (APTES) or dextran. Our approach supports the vision of a simple yet highly sensitive diagnostic platform that could be used for pre-screening patients for a wide range of diseases at point-of-care, thereby relieving the pressure on over-stretched healthcare services.