Proliferating endothelial cells and leukocyte infiltration as prognostic markers in colorectal cancer
Proliferating endothelial cells and leukocyte infiltration as prognostic markers in colorectal cancer
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DOI：
10.1016/j.cgh.2006.08.005
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发表时间：
2006-11-01
影响因子：
12.6
通讯作者：
Griffioen, Arjan W.
中科院分区：
文献类型：
--
作者：
Baeten, Coen I. M.;Castermans, Karolien;Griffioen, Arjan W.
背景和目的：肿瘤中的白细胞浸润取决于血管生成潜力。在这项研究中，我们旨在回顾性研究存档结直肠癌（CRC）组织中的血管生成潜力及其与炎症浸润的数量和成分的关系。方法：对随访 12 年的 117 例 CRC 患者的肿瘤组织中，采用增殖标记物 Ki-67 进行 CD31/CD34 双染色来评估微血管密度（MVD）和增殖内皮细胞（EC）。使用瘤周、肿瘤间质和瘤内区域的 CD45、CD3、CD8、CD16、CD20 和 CD68 抗体测定白细胞浸润。结果：增殖的 EC，而非 MVD，与 CRC 的 Dukes 分期和生存相关（P
Background & Aims: Leukocyte infiltration in tumors is dependent on angiogenic potential. in this study we aimed to retrospectively investigate the angiogenic potential in archival colorectal cancer (CRC) tissues and its relationship to amount and composition of the inflammatory infiltrate. Methods: in tumor tissues of 117 CRC patients with a 12-year follow-up, microvessel density (MVD) and proliferating endothelial cells (ECs) were assessed by CD31/CD34 double staining with the proliferation marker Ki-67. Leukocyte infiltration was determined by using CD45, CD3, CD8, CD16, CD20, and CD68 antibodies in peritumoral, tumor stroma, and intratumoral areas. Results: Proliferating ECs, but not MVD, are correlated to Dukes' stage and survival in CRC (P