Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family
Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family
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DOI：
10.1016/j.ygeno.2004.09.010
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发表时间：
2005-01-01
期刊：
影响因子：
4.4
通讯作者：
Sanai, Y
中科院分区：
文献类型：
--
作者：
Ishida, N;Kuba, T;Sanai, Y
我们报道了SLC35核苷酸糖转运蛋白家族新成员SLC35D2的分子克隆。SLC35D2基因定位于染色体9q22.33。SLC35D2 cDNA编码一个疏水蛋白，由337个氨基酸残基和10个假定的跨膜螺旋组成。Northern blot分析显示SLC35D2 mRNA为单个主条带，长度为2.0 kb。SLC35D2定位于高尔基膜，与人类SLC35D1（葡萄糖醛酸/乙酰半乳糖胺转运蛋白）、果蝇边缘连接转运蛋白（frc）和线虫SQV-7转运蛋白相似度约为50%，后两者参与发育和器官发生过程。SLC35D2蛋白在酵母中的异源表达表明，udp - n -乙酰氨基葡萄糖是该转运蛋白底物的候选物。序列相似性、亚细胞定位和转运底物表明，SLC35D2是frc转运体同源物的良好候选者，其通过向边缘n -乙酰氨基葡萄糖转移酶提供底物参与Notch信号系统。我们还描述了人类SLC35基因家族的鉴定和分类。(C) 2004爱思唯尔公司版权所有。
We report the molecular cloning of SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family. The gene SLC35D2 maps to chromosome 9q22.33. SLC35D2 cDNA codes for a hydrophobic protein consisting of 337 amino acid residues with 10 putative transmembrane helices. Northern blot analysis revealed the SLC35D2 mRNA as a single major band corresponding to 2.0 kb in length. SLC35D2 was localized in the Golgi membrane and exhibited around 50% similarity with three nucleotide sugar transporters: human SLC35D1 (UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter), fruitfly fringe connection (frc) transporter, and nematode SQV-7 transporter, the latter two being involved in developmental and organogenetic processes. Heterologous expression of SLC35D2 protein in yeast indicated that UDP-N-acetylglucosamine is a candidate for the substrate(s) of the transporter. The sequence similarity, subcellular localization, and transporting substrate suggest that SLC35D2 is a good candidate for the ortholog of frc transporter, which is involved in the Notch signaling system by providing the fringe N-acetylglucosaminyltransferase with the substrate. We also describe the identification and categorization of the human SLC35 gene family. (C) 2004 Elsevier Inc. All rights reserved.