A novel ethylene responsive factor CitERF13 plays a role in photosynthesis regulation.
A novel ethylene responsive factor CitERF13 plays a role in photosynthesis regulation.
复制标题
DOI：
10.1016/j.plantsci.2016.11.001
复制
发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Xiu-lan Xie;X. Xia;Sheng Kuang;Xi-li Zhang;Xue‐ren Yin;Jingquan Yu;Kun-song Chen
中科院分区：
文献类型：
--
作者：
Xiu-lan Xie;X. Xia;Sheng Kuang;Xi-li Zhang;Xue‐ren Yin;Jingquan Yu;Kun-song Chen
乙烯反应因子（ERFs）是乙烯信号转导途径的重要下游成分，参与调控植物的生长发育和胁迫反应。然而，人们对它在光合作用中的作用知之甚少。在此，我们鉴定了一个乙烯诱导的柑橘ERF基因CitERF 13。CitERF 13在N.烟草叶片的净光合速率显著降低。对PSII和PSI光合活性的进一步检测表明，CitERF 13过表达导致Fv/Fm、Y（II）和Y（I）下降。CitERF 13过表达还显著降低Vc，max，Jmax和AQY，表明卡尔文循环受到抑制。瞬时过表达CitERF 13的叶片中光合相关基因的表达受到不同程度的抑制。CitERF 13在烟草和柑橘中的瞬时过表达均导致叶绿素含量的下降，并且CitERF 13过表达的烟草叶圆片对MV介导的氧化胁迫更敏感。结果表明，CitERF 13可能通过抑制光合作用的光化学活性、CO2羧化能力和叶绿素代谢等多种途径参与光合作用的抑制。
Ethylene responsive factors (ERFs) act as critical downstream components of the ethylene signalling pathway in regulating plant development and stress responses. However little is known about its role in regulation of photosynthesis. Here, we identified an ethylene-inducible ERF gene in citrus,CitERF13. Transient over-expression ofCitERF13inN. tabacumleaves, resulted in a significant decrease in net photosynthetic rate. Closer examination of photosynthetic activity of PSII and PSI indicated thatCitERF13overexpression led to declines ofFv/Fm,Y(II) andY(I). However, change in NPQ was less pronounced.CitERF13overexpression also significantly reducedVc,max,Jmaxand AQY, indicating inhibition of the Calvin cycle. The expression of photosynthesis-related genes was suppressed to a variable extent in leaf blades transiently over-expressingCitERF13.CitERF13transient overexpression in tobacco or citrus both resulted in a decline of Chlorophyll content andCitERF13overexpressing tobacco leaf disc was more susceptible to chlorosis in response to MV-mediated oxidative stress. The results suggest thatCitERF13is potentially involved in suppressing photosynthesis through multiple pathways, for instance, inhibiting photochemical activity of photosynthesis, CO2carboxylation capacity and chlorophyll metabolism.