The Expression of Interferon-Stimulated Gene 15 in Equine Endometrium
The Expression of Interferon-Stimulated Gene 15 in Equine Endometrium
复制标题
DOI：
10.1111/j.1439-0531.2010.01731.x
复制
发表时间：
2011-08-01
影响因子：
1.7
通讯作者：
Troedsson, M. H. T.
中科院分区：
文献类型：
--
作者：
Klein, C.;Scoggin, K. E.;Troedsson, M. H. T.
怀孕的建立严重依赖于一个精确编排的胚胎-母体相互作用，导致一个接受子宫的环境。干扰素刺激蛋白15kda （ISG15）在怀孕期间的上调已在许多物种中被描述，并被假设为使子宫接受妊娠发育的分子库的一部分。本研究检测了黄体期第14天、妊娠第14天和第50天马子宫内膜中ISG15和ISG15酰化相关酶的mRNA和蛋白水平表达。ISG15 mRNA在妊娠第14天的表达量比妊娠第14天高2.63倍，而妊娠第50天的mRNA丰度与妊娠第14天相比没有变化。使用抗ISG15抗体进行Western blot分析，可以观察到几个更高分子量的条带，代表与ISG15偶联的蛋白质。未检测到空闲的ISG15。ISG15反应蛋白的模式不同于在非子宫样本中观察到的。免疫组化显示，ISG15反应蛋白主要定位于管腔上皮细胞和腺上皮细胞，基质细胞染色较弱。综上所述，isg15结合蛋白在马子宫内膜的表达在排卵后14天和妊娠后第50天无显著差异。据推测，在子宫内膜组织中表达的独特的isg15化蛋白亚群有助于正常的细胞功能，并且与其他物种不同，isg15结合蛋白的修饰并不是母马孕母相互作用的积极贡献者。
Establishment of pregnancy is critically dependent upon a precisely orchestrated embryo-maternal interaction leading to a receptive uterine environment. The up-regulation of the interferon-stimulated protein 15 kDa (ISG15) during pregnancy has been described in various species and has been hypothesized to be part of the molecular repertoire that makes the uterus receptive to conceptus development. In the current study, the expression of ISG15 and enzymes involved in ISG15ylation was examined at the mRNA and protein level in equine endometrium at Day 14 of the luteal phase and at Day 14 and 50 of pregnancy. ISG15 mRNA showed a 2.63-fold higher expression at Day 14 of pregnancy when compared to Day 14 of the cycle, while mRNA abundance at Day 50 of pregnancy was unchanged compared to Day 14 of the cycle. Upon Western blot analysis using anti-ISG15 antibody, several higher molecular weight bands could be observed, representing proteins conjugated to ISG15. No free ISG15 could be detected. The pattern of ISG15 reactive proteins differed from those observed in non-uterine samples. Upon immunohistochemistry, ISG15 reactive proteins located primarily to luminal and glandular epithelial cells, while stromal cells showed weaker staining. In conclusion, the expression of ISG15-conjugated proteins in equine endometrium did not differ between cyclic and pregnant 14 days after ovulation and Day 50 of pregnancy. It is hypothesized that the unique subset of ISG15ylated proteins expressed in endometrial tissue contributes to normal cellular function and that, unlike other species, the modification of ISG15-conjugated proteins is not an active contributor to conceptus-maternal interaction in the mare.