Ribosomal proteins L1, L17 and L27 from Escherichia coli localized at single sites on the large subunit by immune electron microscopy.
Ribosomal proteins L1, L17 and L27 from Escherichia coli localized at single sites on the large subunit by immune electron microscopy.
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通过免疫电子显微镜观察，来自大肠杆菌的核糖体蛋白 L1、L17 和 L27 位于大亚基的单个位点。
DOI：
10.1016/0022-2836(81)90462-9
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发表时间：
1981
影响因子：
5.6
通讯作者：
Strycharz,WA
中科院分区：
文献类型：
--
作者：
Lake,JA;Strycharz,WA
使用针对这些蛋白质的抗体，通过免疫电子显微镜确定了大肠杆菌核糖体蛋白 L1、L17 和 L27 的三维位置。根据在两个特征投影中观察到的免疫球蛋白G附着位置，可以确定这三种蛋白质位于大亚基表面不同区域的单个位点。在准对称投影中，L1 映射在“L 7 L 12 茎”对面的一侧，称为 L1 脊；蛋白质 L17 定位在与“中央突起”相对的亚基底部（朝向亚基的 L 7 L 12 侧）；蛋白质 L27 发现于中央突起（位于 L 7 L 12 柄的远端）。在不对称投影中，L1和L27蛋白位于与小亚基收缩的亚基表面，L17蛋白位于远离小亚基的亚基表面；即在大亚基的细胞质表面上。当免疫球蛋白 G 分子与其特定蛋白质一起预吸收时，所有三个位点上的抗体结合都被消除。
Three-dimensional locations have been determined for Escherichia coli ribosomal proteins L1, L17 and L27 by immune electron microscopy using antibodies directed against these proteins. From the positions of immunoglobulin G attachment, observed in two characteristic projections, it was determined that these three proteins are located at single sites in different regions on the surface of the large subunit. In the quasisymmetric projection, L1 maps on the side opposite the “L 7 L 12 stalk,” named the L1 ridge; protein L17 maps at the base of the subunit opposite the “central protuberance”(toward the L 7 L 12 side of the subunit); and protein L27 is found on the central protuberance (on the side distal to the L 7 L 12 stalk). In the asymmetric projection, proteins L1 and L27 are found on the surface of the subunit contracting the small subunit and protein L17 is on the surface of the subunit distal to the small subunit; ie on the cytoplasmic surface of the large subunit. Antibody binding at all three sites was eliminated when the immunoglobulin G molecules were preabsorbed with their specific proteins.