Early embryo development in the Siberian hamster (Phodopus sungorus).
Early embryo development in the Siberian hamster (Phodopus sungorus).
复制标题
西伯利亚仓鼠（Phodopus sungorus）的早期胚胎发育。
DOI：
10.1002/mrd.1080270307
复制
发表时间：
1990
影响因子：
2.5
通讯作者：
Caprio,TL
中科院分区：
文献类型：
--
作者：
Nieder,GL;Caprio,TL
对西伯利亚仓鼠（Phodopus sungorus）植入前胚胎的体内和体外发育进行了检查。研究发现体内早期发育的时间比报道的金仓鼠要慢。卵裂阶段、空化和透明带孵化的进展发生较晚，囊胚形成于第 4 天下午开始，子宫附着于第 5 天早期发生。在体外，第 4 天收集桑葚胚和早期囊胚并在含血清培养基中培养，形成扩大的囊胚，其中一些开始从透明带孵化。随着培养时间的延长，囊胚附着并形成滋养层生长物。生长的特征是小细胞从囊胚中最初迁移，随后形成滋养层巨细胞片。仓鼠和小鼠生长形态的差异表明，与西伯利亚仓鼠进行进一步的比较研究可能是有用的。
Development of preimplantation embryos of the Siberian hamster (Phodopus sungorus) in vivo and in vitro was examined. The timing of early development in vivo was found to be slower than that reported for the golden hamster. Progression through the cleavage stages, cavitation, and hatching from the zona pellucida occurred later, with blastocyst formation beginning on the afternoon of day 4 and uterine attachment occurring early on day 5. In vitro, morulae, and early blastocysts collected on day 4 and cultured in serum‐containing medium formed expanded blastocysts and some began to hatch from the zona pellucida. With extended culture, blastocysts attached and formed trophoblast outgrowths. Outgrowth was characterized by an initial migration of small cells from the blastocyst, followed by formation of a sheet of trophoblast giant cells. Differences in the morphology of outgrowth between the hamster and mouse suggest that further comparative studies with the Siberian hamster may be useful.