Compact, common path quantitative phase microscopic techniques for imaging cell dynamics
Compact, common path quantitative phase microscopic techniques for imaging cell dynamics
复制标题
用于细胞动力学成像的紧凑、通用路径定量相显微技术
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
B. Javidi
中科院分区：
文献类型：
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作者：
A. Anand;P. Vora;S. Mahajan;V. Trivedi;V. Chhaniwal;A. Singh;R. Leitgeb;B. Javidi
使用可见电磁辐射的显微镜可用于研究各种环境中的活细胞。但是，明场显微镜仅提供单个物面处的二维（2D）强度分布。检索物体高度/厚度信息的方法之一是采用定量相位显微镜（QPM）技术。干涉测量QPM技术广泛用于此。数字全息显微术（DHM）是用于定量三维（3D）成像的最先进方法之一。通常，它是在两个梁的几何形状，这是易于机械振动。但是要研究像红细胞这样的物体的动力学，人们需要比物体波动更好的时间稳定性，而双光束几何学无法提供。克服这一障碍的一种方法是使用自参考技术，其中物体光束的一部分将充当参考光束。在这里，自参考QPM技术的发展沿着的结果一起描述。
Microscopy using visible electromagnetic radiation can be used to investigate living cells in various environments. But bright field microscopy only provides two-dimensional (2D) intensity distribution at a single object plane. One of the ways to retrieve object height/thickness information is to employ quantitative phase microscopic (QPM) techniques. Interferometric QPM techniques are widely used for this. Digital holographic microscopy (DHM) is one of the state-of-the-art methods for quantitative three-dimensional (3D) imaging. Usually it is implemented in two-beam geometry, which is prone to mechanical vibrations. But to study dynamics of objects like red blood cells, one needs temporal stability much better than the fluctuations of the object, which the two-beam geometry fails to deliver. One way to overcome this hurdle is to use self-referencing techniques, in which a portion of the object beam will act as the reference beam. Here the development of self-referencing QPM techniques is described along with the results.
影响因子：
3.6
作者：
Shaked NT;Rinehart MT;Wax A
通讯作者：
Wax A
影响因子：
3.5
作者：
Popescu, Gabriel;Badizadegan, Kamran;Feld, Michael S.
通讯作者：
Feld, Michael S.