Expression of decorin throughout the murine hair follicle cycle: hair cycle dependence and anagen phase prolongation
Expression of decorin throughout the murine hair follicle cycle: hair cycle dependence and anagen phase prolongation
复制标题
DOI：
10.1111/exd.12441
复制
发表时间：
2014-07-01
影响因子：
3.6
通讯作者：
Lu, Zhong-Fa
中科院分区：
文献类型：
--
作者：
Jing, Jing;Wu, Xian-jie;Lu, Zhong-Fa
核心蛋白聚糖是富含亮氨酸的小蛋白聚糖（SLRP）家族的典型成员，其参与许多生物过程。核心蛋白聚糖作为SLRP的代表，在毛囊形态发生中的作用尚未阐明。我们提出了我们的初步研究结果在诱导小鼠毛囊（HF）周期的核心蛋白聚糖的表达模式。结果发现，核心蛋白聚糖表达仅限于表皮，外根鞘和皮脂腺在生长期阶段，这与上调的核心蛋白聚糖mRNA和蛋白质表达在脱毛鼠背部皮肤。此外，我们使用了一种功能性的方法来研究重组人核心蛋白聚糖（rhDecorin）的影响，通过皮肤注射到HFs在不同的小鼠毛发周期阶段。在毛发生长期将rhDecorin（100 μ g/ml）局部注射到脱毛的C57 BL/6小鼠的皮下组织中延迟了毛发退化期的进展。相比之下，在休止期期间注射rhDecorin引起毛发生长期的过早开始，如通过以下参数的评估所证明的：（i）毛干长度，（ii）毛囊球直径，（iii）毛囊循环评分和（iv）毛囊期百分比。综上所述，我们的研究结果表明，核心蛋白聚糖可以调节卵泡周期和形态发生。此外，这项研究还提供了深入了解毛囊上皮间质相互作用的分子控制机制。
Decorin is a prototypical member of the small leucine-rich proteoglycan (SLRP) family, which is involved in numerous biological processes. The role of decorin, as a representative SLRP, in hair follicle morphogenesis has not been elucidated. We present our initial findings on decorin expression patterns during induced murine hair follicle (HF) cycles. It was found that decorin expression is exclusively restricted to the epidermis, outer root sheath and sebaceous glands during the anagen phase, which correlates with the upregulation of decorin mRNA and protein expression in depilated murine dorsal skin. Furthermore, we used a functional approach to investigate the effects of recombinant human decorin (rhDecorin) via cutaneous injection into HFs at various murine hair cycle stages. The local injection of rhDecorin (100 mu g/ml) into the hypodermis of depilated C57BL/6 mice at anagen delayed catagen progression. In contrast, rhDecorin injection during the telogen phase caused the premature onset of anagen, as demonstrated by the assessment of the following parameters: (i) hair shaft length, (ii) follicular bulbar diameter, (iii) hair follicle cycling score and (iv) follicular phase percentage. Taken together, our results suggest that decorin may modulate follicular cycling and morphogenesis. In addition, this study also provides insight into the molecular control mechanisms governing hair follicular epithelial-mesenchymal interactions.