Detection of neovessels in atherosclerotic plaques of rabbits using dynamic contrast enhanced MRI and 18F-FDG PET
Detection of neovessels in atherosclerotic plaques of rabbits using dynamic contrast enhanced MRI and 18F-FDG PET
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DOI：
10.1161/atvbaha.108.166173
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发表时间：
2008-07-01
影响因子：
8.7
通讯作者：
Fayad, Zahi A.
中科院分区：
文献类型：
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作者：
Calcagno, Claudia;Cornily, Jean-Christophe;Fayad, Zahi A.
目的：动脉粥样硬化中炎性细胞和新生血管的相关性被认为是高危活动性病变的组织学标志。因此，开发和验证非侵入性成像技术，以检测动脉粥样硬化中的炎症和新血管生成将是主要的临床兴趣。我们的目的是测试两种技术，即黑血动态增强磁共振成像(DCE-MRI)和18F-氟代脱氧葡萄糖(18F-FDG)PET，以量化动脉粥样硬化模型中以斑块新生血管含量表示的炎症。方法和结果-通过2次内皮磨损和4个月的高脂饮食，在10只兔的主动脉形成动脉粥样硬化斑块。6只兔在注射Gd-DTPA时进行了MRI检查，4只兔在注射18F-FDG后进行了PET成像。我们发现动脉粥样硬化斑块中的新生血管计数与(1)DCE-MRI评估的Gd-DTPA摄取参数(r=0.89，P=0.016)和(2)PET评估的18F-FDG摄取(r=0.5，P=0.103)呈正相关。结论DCE-MRI和18F-FDGPET可用于评价兔动脉斑块的炎症状态。这些非侵入性影像检查可作为评估病变预后和监测抗血管生成治疗的临床工具。
Objective-The association of inflammatory cells and neovessels in atherosclerosis is considered a histological hallmark of high-risk active lesions. Therefore, the development and validation of noninvasive imaging techniques that allow for the detection of inflammation and neoangiogenesis in atherosclerosis would be of major clinical interest. Our aim was to test 2 techniques, black blood dynamic contrast enhanced MRI (DCE-MRI) and 18-fluorine-fluorodeoxyglucose (18F-FDG) PET, to quantify inflammation expressed as plaque neovessels content in a rabbit model of atherosclerosis.Methods and Results-Atherosclerotic plaques were induced in the aorta of 10 rabbits by a combination of 2 endothelial abrasions and 4 months hyperlipidemic diet. Six rabbits underwent MRI during the injection of Gd-DTPA, whereas 4 rabbits were imaged after injection of 18F-FDG with PET. We found a positive correlation between neovessels count in atherosclerotic plaques and (1) Gd-DTPA uptake parameters evaluated by DCE-MRI (r=0.89, P=0.016) and (2) 18F-FDG uptake evaluated by PET (r=0.5, P=0.103 after clustered robust, Huber-White, standard errors analysis).Conclusion-DCE-MRI and 18F-FDG PET may allow for the evaluation of inflammation in atherosclerotic plaques of rabbits. These noninvasive imaging modalities could be proposed as clinical tools in the evaluation of lesion prognosis and monitoring of anti-angiogenic therapies.