Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties
Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties
复制标题
DOI：
10.1016/j.msec.2015.11.052
复制
发表时间：
2016-03-01
影响因子：
7.9
通讯作者：
Kup, Fatma Ozturk
中科院分区：
文献类型：
--
作者：
Duman, Fatih;Ocsoy, Ismail;Kup, Fatma Ozturk
在这项研究中，我们报告的氧化铜纳米粒子（CuO NPs）的合成使用药用植物（母菊洋甘菊）花提取物作为还原剂和封端剂，并研究其抗氧化活性和与质粒DNA（pBR 322）的相互作用。采用紫外-可见光谱、傅里叶变换红外光谱、动态光散射、X射线衍射、能谱和扫描电子显微镜对纳米CuO进行了表征。CuO纳米颗粒呈近单分散的球形，粒径约为140 nm。CuO NP的UV-Vis吸收光谱在285和320 nm附近产生宽峰。红外光谱分析表明纳米氧化铜表面存在官能团。XRD图谱显示纳米粒子为面心立方晶体。由于在植物提取物中存在带负电荷的官能团，Zeta电位值测量为20 mV。此外，我们证明了CuO纳米颗粒的浓度依赖性抗氧化活性及其与质粒DNA的相互作用。我们假设CuO纳米颗粒既切割又破坏DNA双螺旋结构。(C)2015 Elsevier B. V.版权所有。
In this study, we report the synthesis of copper oxide nanoparticles (CuO NPs) using a medicinal plant (Matricaria chamomilla) flower extract as both reducing and capping agent and investigate their antioxidant activity and interaction with plasmid DNA (pBR322). The CuO NPs were characterized using Uv-Vis spectroscopy, FT-IR (Fourier transform infrared spectroscopy), DLS (dynamic light scattering), XRD (X-ray diffraction), EDX (energy-dispersive X-ray) spectroscopy and SEM (scanning electron microscopy). The CuO NPs exhibited nearly mono-distributed and spherical shapes with diameters of 140 nm size. UV-Vis absorption spectrum of CuO NPs gave a broad peak around 285 and 320 nm. The existence of functional groups on the surface of CuO NPs was characterized with FT-IR analysis. XRD pattern showed that the NPs are in the form of a face-centered cubic crystal. Zeta potential value was measured as 20 mV due to the presence of negatively charged functional groups in plant extract. Additionally, we demonstrated concentration-dependent antioxidant activity of CuO NPs and their interaction with plasmid DNA. We assumed that the CuO NPs both cleave and break DNA double helix structure. (C) 2015 Elsevier B.V. All rights reserved.