HEALING OF LARGE SEGMENTAL DEFECTS IN RAT FEMURS IS AIDED BY RHBMP-2 IN PLGA MATRIX
HEALING OF LARGE SEGMENTAL DEFECTS IN RAT FEMURS IS AIDED BY RHBMP-2 IN PLGA MATRIX
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DOI：
10.1002/jbm.820281005
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发表时间：
1994-10-01
期刊：
影响因子：
--
通讯作者：
HAYES, WC
中科院分区：
文献类型：
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作者：
LEE, SC;SHEA, M;HAYES, WC
当在适当的载体中配制时，重组人骨形态发生蛋白-2（rhBMP-2）可用于增强先天性或获得性骨病变的修复。聚[D，L-（丙交酯-乙交酯）]（PLGA）已被证明是rhBMP-2的有效载体。我们在大鼠原位模型中研究了几种粒径的PLGA和几种剂量的rhBMP-2。我们还研究了纤维蛋白溶解抑制剂，β-氨基己酸（EACA），对愈合反应的影响。我们的数据表明，较高剂量的rhBMP-2导致失效扭矩增加（408 +/- 70 N-mm或完整值的60%）和愈合发生率较高（100%）。用较小粒径的PLGA处理的股骨中的诱导骨实现了最大的扭转刚度和强度。rhBMP-2的存在是新骨形成所必需的，但EACA的存在并没有改变这些结果;使用PLGA载体似乎增加了骨强度和刚度。事实上，在PLGA中加入更高剂量的rhBMP-2，新骨的硬度与完整对照相同（64 +/- 20 N-mm/度[完整股骨]对比45 +/- 10 N-mm/度[小PLGA中的中等剂量]、61 +/- 17 N-mm/度[小PLGA中的高剂量]和36 +/- 11 N-mm/度[大PLGA中的中等剂量]; P > 0.05）。总之，PLGA植入rhBMP-2有效地帮助愈合大鼠股骨大段缺损。(C)John Wiley & Sons，Inc.
Recombinant human bone morphogenetic protein-2 (rhBMP-2) can be used to enhance the repair of congenital or acquired bone pathologies when formulated in the appropriate carrier. Poly [D,L-(lactide-co-glycolide)] (PLGA) has been shown to be an effective carrier of rhBMP-2. We investigated several particle sizes PLGA and several doses of rhBMP-2 in a rat orthotopic model. We also investigated the effects of a fibrinolytic inhibitory agent, epsilon aminocaproic acid (EACA), on the healing response. Our data indicate that higher doses of rhBMP-2 resulted in increased failure torque (408 +/- 70 N-mm or 60% of the intact value) and higher incidence of union (100%). The induced bone in femurs treated with the smaller particle size PLGA achieved the greatest torsional stiffness and strength. The presence of rhBMP-2 was necessary for new bone to form, but the presence of EACA did not change these results; the use of the PLGA carrier appeared to increase bone strength and stiffness. In fact, with higher doses of rhBMP-2 in PLGA, the stiffness of the new bone was equal to that of intact controls (64 +/- 20 N-mm/deg [intact femurs] versus 45 +/- 10 N-mm/degree [medium dose in small PLGA], 61 +/- 17 N-mm/degree [high dose in small PLGA], and 36 +/- 11 N-mm/degree [medium dose in large PLGA]; P > .05). In conclusion, PLGA implanted with rhBMP-2 effectively aided in healing large segmental defects in rat femurs. (C) 1994 John Wiley & Sons, Inc.