A simple and rapid immunochromatographic strip test for detecting antibody to porcine reproductive and respiratory syndrome virus
A simple and rapid immunochromatographic strip test for detecting antibody to porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.jviromet.2008.05.029
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发表时间：
2008-09-01
影响因子：
3.1
通讯作者：
Oh, JinSik
中科院分区：
文献类型：
--
作者：
Cui, Shangjin;Zhou, Shenghua;Oh, JinSik
猪繁殖与呼吸综合征（Porcine Reproductive and Respiratory Syndrome，PRRS）是世界范围内最具经济意义的猪病之一。目前检测猪繁殖与呼吸综合征病毒（PRRSV）抗体主要采用酶联免疫吸附试验、血清中和试验相结合的方法。免疫过氧化物酶单层试验，间接免疫荧光抗体试验。这些方法很耗时，需要由训练有素的技术人员操作的专门设备。本研究的目的是评估一种简单的试纸条检测（基于层析和免疫金系统）特异性检测猪血清中的PRRSV抗体。本试验采用大肠杆菌表达的病毒重组膜蛋白抗原结合重组核衣壳蛋白作为捕获蛋白，检测猪繁殖与呼吸综合征病毒（PRRSV）抗体。通过免疫层析试纸条检测进行了比较，通过一个标准的检测，市售的间接酶联免疫吸附试验和免疫过氧化物酶单层检测。免疫层析试纸条检测已知含有PRRSV抗体的血清中的抗体，在95.7%的灵敏度从实验感染的猪的样品和98.6%的灵敏度的临床血清样品。对于不含抗PRRSV抗体的血清，临床和实验血清样品的特异性分别为97.8%和98.2%。(C)2008 Elsevier B.V.保留所有权利。
Porcine reproductive and respiratory syndrome is rapidly gaining worldwide importance as one of the most economically significant diseases of swine. The antibody of Porcine reproductive and respiratory syndrome virus (PRRSV) is detected currently by the combined use of an enzyme-linked immunosorbent assay, serum neutralization test. immunoperoxidase monolayer assay, indirect immunofluorescent antibody test. These methods are time-consuming and require specialized equipment operated by trained technicians. The purpose of this study was to evaluate a simple strip assay (based on a chromatographic and immunogold system) for specific detection of PRRSV antibody in swine sera. This "immunochromatographic strip" test uses Escherichia coli-expressed viral recombinant membrane protein antigen in combination with recombinant nucleocapsid protein as capture protein for detecting antibodies against PRRSV, In this study, the performance of this assay was evaluated with sera from both clinical samples and experimentally infected piglets. Detection by immunochromatographic strip test was compared with detection by a standard, available commercially, indirect enzyme-linked immunosorbent assay and an immunoperoxidase monolayer assay. The immunochromatographic test strip detected antibodies in sera known to contain antibodies to PRRSV in 95.7% sensitivity of samples from pigs infected experimentally and 98.6% sensitivity of clinical serum samples. For sera that did not contain antibodies to PRRSV, the specificity was 97.8% and 98.2% for clinical and experimental serum samples, respectively. (C) 2008 Elsevier B.V. All rights reserved.