Development of Near-Isogenic Lines in a Parthenogenetically Reproduced Thrips Species, Frankliniella occidentalis.
Development of Near-Isogenic Lines in a Parthenogenetically Reproduced Thrips Species, Frankliniella occidentalis.
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孤雌生殖蓟马种（Frankliniella occidentalis）近源系的发育
DOI：
10.3389/fphys.2017.00130
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发表时间：
2017
影响因子：
4
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Yuan G;Wan Y;Li X;He B;Zhang Y;Xu B;Wang S;Xie W;Zhou X;Wu Q
虽然近等基因系（near-isogenic lines, NILs）可以标准化个体之间的遗传背景，但它从未应用于孤雌生殖动物。本研究通过多轮回交和spinosad筛选，在具有单倍体-二倍体生殖系统的西部花蓟马Frankliniella occidentalis (Pergande) （Thysanoptera: Thripidae）中获得了具有spinosad抗性的NILs。得到的西芽孢杆菌NIL-R菌株的抗性比保持在3万倍以上，与其亲本抗性菌株Spin-R相当。更重要的是，西方F. occidentalis NIL-R与其敏感亲本菌株Ivf03的遗传相似性为98.90%。通过开发该工具集，我们能够分离个体抗性，并促进韧皮部摄食节肢动物对杀虫剂抗性的机制研究，这是一组毁灭性的害虫物种，可以有性繁殖和无性繁殖。
Although near-isogenic lines (NILs) can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant NILs in the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), with a haplo-diploid reproduction system. The resultant F. occidentalis NIL-R strain maintained a resistance ratio over 30,000-fold, which was comparable to its parental resistant strain, Spin-R. More importantly, F. occidentalis NIL-R shared 98.90% genetic similarity with its susceptible parental strain Ivf03. By developing this toolset, we are able to segregate individual resistance and facilitate the mechanistic study of insecticide resistances in phloem-feeding arthropods, a group of devastating pest species reproducing sexually as well as asexually.