Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients
Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients
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DOI：
10.1093/annonc/mdh017
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发表时间：
2004-01-01
影响因子：
50.5
通讯作者：
Voest, EE
中科院分区：
文献类型：
--
作者：
Beerepoot, LV;Mehra, N;Voest, EE
背景：越来越多的临床前研究表明，循环内皮细胞在血管新生和肿瘤生长中起着重要作用。CEC在人类癌症进展中的作用很少被研究。因此，我们分析了癌症患者外周血中的CEC。此外，我们在实验模型中将这些患者的CEC水平与已知可动员CEC的血浆细胞因子水平相关联。患者和方法：通过使用磁珠与针对泛内皮细胞标志物CD146的抗体偶联，从癌症患者的全血中分离、定量和培养可存活的CEC。钙调素AM染色观察细胞存活情况。结果：95例进展期癌症患者的CEC平均是正常人的3.6倍(P<0.05)。
Background: There is accumulating evidence from preclinical studies that circulating endothelial cells (CECs) play an important role in neovascularization and tumor growth. The role of CECs in human cancer progression is sparsely investigated. We therefore analyzed CECs in peripheral blood of cancer patients. In addition, we correlated CEC levels in these patients with plasma levels of cytokines that are known to mobilize CECs in experimental models.Patients and methods: Viable CECs were isolated, quantified and cultured from cancer patients' whole blood by using magnetic beads coupled to an antibody directed against CD146, a pan-endothelial marker. Viable cells were visualized by calceinAM staining. Positive staining for specific endothelial cell markers [i.e. von Willebrand factor, CD31, vascular endothelial cell growth factor (VEGF) receptor-2] was used to confirm the endothelial phenotype.Results: Cancer patients with progressive disease (95 patients) had on average 3.6-fold more CECs than healthy subjects (46 patients, P