cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization.
cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization.
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DOI：
10.1128/mbio.00966-18
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发表时间：
2018-07-10
期刊：
影响因子：
6.4
通讯作者：
Grainger DC
中科院分区：
文献类型：
--
作者：
Manneh-Roussel J;Haycocks JRJ;Magán A;Perez-Soto N;Voelz K;Camilli A;Krachler AM;Grainger DC
霍乱弧菌这种细菌原产于水生环境，可以改变生活方式，从而导致人类疾病。生活方式转换需要调节群体感应、肠道定植和毒素产生的遗传系统。这种调节大部分发生在基因表达水平，并受转录因子控制。在这项工作中，我们绘制了cAMP受体蛋白（CRP）和RNA聚合酶在霍乱弧菌基因组中的结合。我们表明，CRP是一个不可分割的组成部分的监管网络，控制生活方式的转换。集中在一个位点的毒素运输所必需的，我们证明CRP依赖的调节基因表达在响应主机殖民。对CRP靶向基因的进一步研究表明，这种行为是常见的。因此，CRP是许多霍乱弧菌基因响应生活方式改变的关键调节因子。霍乱是一种由霍乱弧菌引起的传染病。这种细菌以引起人类疾病而闻名，最常见于水生生态系统中。因此，人类在摄入被霍乱弧菌污染的食物或水后感染霍乱。水生环境和人类宿主之间的过渡触发了生活方式的转变，涉及霍乱弧菌基因表达模式的重新编程。这一过程由转录因子网络控制。在本文中，我们表明，环磷酸腺苷受体蛋白（CRP）是一个关键的调节器的霍乱弧菌基因表达的生活方式的变化。
The bacterium Vibrio cholerae is native to aquatic environments and can switch lifestyles to cause disease in humans. Lifestyle switching requires modulation of genetic systems for quorum sensing, intestinal colonization, and toxin production. Much of this regulation occurs at the level of gene expression and is controlled by transcription factors. In this work, we have mapped the binding of cAMP receptor protein (CRP) and RNA polymerase across the V. cholerae genome. We show that CRP is an integral component of the regulatory network that controls lifestyle switching. Focusing on a locus necessary for toxin transport, we demonstrate CRP-dependent regulation of gene expression in response to host colonization. Examination of further CRP-targeted genes reveals that this behavior is commonplace. Hence, CRP is a key regulator of many V. cholerae genes in response to lifestyle changes. Cholera is an infectious disease that is caused by the bacterium Vibrio cholerae. Best known for causing disease in humans, the bacterium is most commonly found in aquatic ecosystems. Hence, humans acquire cholera following ingestion of food or water contaminated with V. cholerae. Transition between an aquatic environment and a human host triggers a lifestyle switch that involves reprogramming of V. cholerae gene expression patterns. This process is controlled by a network of transcription factors. In this paper, we show that the cAMP receptor protein (CRP) is a key regulator of V. cholerae gene expression in response to lifestyle changes.