Developmental changes in the responsiveness of hypothalamic ER alpha mRNA levels to food deprivation.
Developmental changes in the responsiveness of hypothalamic ER alpha mRNA levels to food deprivation.
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发表时间：
2013
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通讯作者：
T. Iwasa;T. Matsuzaki;R. Kinouchi;G. Gereltsetseg;M. Murakami;A. Kuwahara;T. Yasui;M. Irahara
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作者：
T. Iwasa;T. Matsuzaki;R. Kinouchi;G. Gereltsetseg;M. Murakami;A. Kuwahara;T. Yasui;M. Irahara
目的雌激素在摄食和能量平衡中起重要作用，而雌激素在食欲和能量平衡中的关键作用是通过下丘脑雌激素受体（ER）α介导的。在营养不良的啮齿动物中，下丘脑ER α mRNA表达降低。一些下丘脑因素对负能量平衡的反应在新生儿早期发展。设计本研究旨在观察禁食诱导雌性大鼠下丘脑ER α mRNA表达的发育变化。结果禁食12 h或24 h后，ER α mRNA表达在出生后15 d和25 d均降低，但在出生后5 d无明显变化。出生后第25天大鼠的血清雌二醇水平没有改变禁食。虽然血清瘦素水平被抑制禁食在所有年龄段，下丘脑ER α mRNA表达在出生后第25天没有改变瘦素管理后，24小时的快速。结论：这些数据表明，下丘脑ER α对负能量平衡的敏感性可能不会在新生儿早期建立，并在出生后15天发展。瘦素水平的降低可能不参与营养不良状态下下丘脑ER α mRNA表达的改变。
OBJECTIVE Estrogen plays an important role in feeding and energy balance, and the critical role of estrogen in the control of appetite and energy balance is mediated by hypothalamic estrogen receptor (ER) alpha. In undernourished rodents, hypothalamic ER alpha mRNA expression are decreased. Responses of some hypothalamic factors to negative energy balance develop during the early neonatal period. DESIGN In this study, we examined the developmental changes of fasting-induced alterations in hypothalamic ER alpha mRNA expression in female rats. RESULTS ER alpha mRNA expression was reduced after a 12-h or 24-h fast at postnatal days 15 and 25, but not at day 5. Serum estradiol levels in postnatal day 25 rats were not changed by fasting. Although serum leptin levels were suppressed by fasting at all ages, hypothalamic ER alpha mRNA expression at postnatal day 25 was not changed by leptin administration after a 24-h fast. CONCLUSIONS These data show that the sensitivity of hypothalamic ER alpha to negative energy balance may not be established in the early neonatal period, and that it develops by postnatal day 15. Decreased leptin levels might not be involved in the alterations of hypothalamic ER alpha mRNA expression in the undernourished condition.