Role of microRNA-15a-5p in the atherosclerotic inflammatory response and arterial injury improvement of diabetic by targeting FASN (Retracted Article)
Role of microRNA-15a-5p in the atherosclerotic inflammatory response and arterial injury improvement of diabetic by targeting FASN (Retracted Article)
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DOI：
10.1042/bsr20181852
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发表时间：
2019-07-02
影响因子：
4
通讯作者：
Ma, Fu-zhe
中科院分区：
文献类型：
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作者：
Liu, Yang;Liu, Ling-yun;Ma, Fu-zhe
本研究旨在探讨miR-15a-5p通过调节脂肪酸合成酶(FASN)在糖尿病大鼠动脉粥样硬化(AS)炎症反应和动脉损伤改善中的作用机制。首先，应用生物信息学工具评估miRNAs和与AS相关的基因，并使用双荧光素酶报告基因分析法测量miRNAs和FASN之间的靶向关系。随后建立糖尿病AS大鼠模型，将存活大鼠分为阴性对照组(NC)、miR-15a-5p模拟组、miR-15a-5p抑制剂组、sh-FASN组和miR-15a-5p+sh-FASN组。然后进行一系列实验，以检测每组的AS程度。结果表明，抑制miR-15a-5p后，与NC组相比，抑制miR-15a-5p后大鼠动脉组织中C-反应蛋白(CRP)、白介素6(IL-6)、细胞间黏附分子-1(ICAM-1)的表达增加，血清低密度脂蛋白胆固醇(LDL-C)、血糖(BG)、甘油三酯(TG)、总胆固醇(TC)、同型半胱氨酸(Hcy)水平升高，高密度脂蛋白胆固醇(HDLc)水平降低，脂肪储存面积扩大(P
The present study aims to investigate the mechanism of miR-15a-5p in the atherosclerotic (AS) inflammatory response and arterial injury improvement in diabetic rats by regulating fatty acid synthase (FASN). Initially, bioinformatics tools were applied to evaluate miRNAs and genes correlating with AS, and the target relation between miRNAs and FASN was measured using the Dual-Luciferase Reporter Assay. Subsequently the diabetic AS rat model was established and the surviving rats were divided into: negative control (NC), miR-15a-5p mimic, miR-15a-5p inhibitor, sh-FASN and miR-15a-5p + sh-FASN groups. Then a series of experiments were performed to examine the degree of AS in each group. The results revealed that compared with the NC group, the expressions of C-reactive protein (CRP), interleukin 6 (IL-6), intercellular cell adhesion molecule-1 (ICAM1) in rat arterial tissue, as well as the levels of low-density lipoprotein cholesterol (LDL-C), blood glucose (BG), triglycerides (TG), total cholesterol (TC) and Homocysteine (Hcy) in rat serum, were increased after inhibiting miR-15a-5p, while the level of high-density lipoprotein cholesterol (HDL-C) was decreased and the fat storage area was enlarged after this treatment (P