Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms
Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms
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DOI：
10.1016/s0092-8674(00)80466-3
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发表时间：
1997-12-12
期刊：
影响因子：
64.5
通讯作者：
Hogness, DS
中科院分区：
文献类型：
--
作者：
Bender, M;Imam, FB;Hogness, DS
类固醇激素蜕皮激素通过三种异二聚体受体指导果蝇变态，所述异二聚体受体根据由EcR基因编码的三种蜕皮激素受体亚型（EcR-A、EcR-B1或EcR-B2）中的哪一种被孤儿核受体USP激活而不同。我们已经确定和分子映射两类EcR突变：那些特定于EcR-B1的解偶联变态，和胚胎致死突变映射到编码DNA和配体结合域的共同序列。在幼虫唾液腺中，EcR-B1的缺失导致蜕皮激素诱导的基因的活化丧失。EcR-B1、EcR-B2和EcR-A在这些突变体腺体中的可比较的转基因表达分别导致该损失的完全、部分和无修复。
The steroid hormone ecdysone directs Drosophila metamorphosis via three heterodimeric receptors that differ according to which of three ecdysone receptor isoforms encoded by the EcR gene (EcR-A, EcR-B1, or EcR-B2) is activated by the orphan nuclear receptor USP. We have identified and molecularly mapped two classes of EcR mutations: those specific to EcR-B1 that uncouple metamorphosis, and embryonic-lethal mutations that map to common sequences encoding the DNA-and ligand-binding domains. In the larval salivary gland, loss of EcR-B1 results in loss of activation of ecdysone-induced genes. Comparable transgenic expression of EcR-B1, EcR-B2, and EcR-A in these mutant glands results, respectively, in full, partial, and no repair of that loss.