CHARACTERIZATION AND PURIFICATION FROM HUMAN-BRAIN OF A HYALURONIC ACID-BINDING GLYCOPROTEIN, HYALURONECTIN
CHARACTERIZATION AND PURIFICATION FROM HUMAN-BRAIN OF A HYALURONIC ACID-BINDING GLYCOPROTEIN, HYALURONECTIN
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DOI：
10.1111/j.1471-4159.1981.tb01672.x
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发表时间：
1981-01-01
影响因子：
4.7
通讯作者：
HALAVENT, C
中科院分区：
文献类型：
--
作者：
DELPECH, B;HALAVENT, C
利用亲和层析和酶标记免疫分析结合亲和吸附，鉴定出一种与透明质酸结合的[人]脑糖蛋白，命名为透明质连蛋白。这种结合可被透明质酸及其水解物所抑制，但不被其他糖胺聚糖或单糖所抑制。制备亲和层析得到的脑酸溶蛋白透明质酸结合蛋白纯度较好。白蛋白污染小于1%，产率高达80%。
Using affinity chromatography and enzyme-labeled immunological assays combined with affinity adsorption, a [human] brain glycoprotein which binds to hyaluronic acid was identified and was named hyaluronectin. This binding was inhibited by hyaluronic acid and by the products of its hydrolysis by hyaluronidase from bovine testis, but was not inhibited by other glycosaminoglycans or by monosaccharides. Preparative affinity chromatography of brain acid-soluble proteins produced hyaluronectin in a good degree of purity. Contamination by albumin was less than 1% and the yield was as high as 80%.