Functional inactivation in the whole population of human V gamma 9/V delta 2 T lymphocytes induced by a nonpeptidic antagonist.
Functional inactivation in the whole population of human V gamma 9/V delta 2 T lymphocytes induced by a nonpeptidic antagonist.
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非肽拮抗剂诱导的整个人 V γ 9/V δ 2 T 淋巴细胞群的功能失活。
DOI：
10.1084/jem.185.1.91
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发表时间：
1997-01-06
期刊：
影响因子：
--
通讯作者：
De Libero G
中科院分区：
文献类型：
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作者：
Bürk MR;Carena I;Donda A;Mariani F;Mori L;De Libero G
在没有主要组织相容性复合物限制的情况下，非肽性化合物刺激携带TCR-γδ的人T细胞。我们报道了其中一种配体，2,3-二磷酸甘油酸（DPG），诱导v γ - 9/Vδ T细胞体外扩增，在重复激活后拮抗相同的细胞群。DPG刺激导致部分早期蛋白酪氨酸磷酸化和v γ - 9/Vδ2中对激动剂配体的长时间但可逆的无反应状态，但在其他T细胞中没有。这些发现表明TCR拮抗是T细胞的普遍现象。然而，与改变多肽拮抗αβ T细胞的克隆特异性相反，所有测试的Vγ9/Vδ2多克隆细胞系和克隆都变得无反应，这一事实可能与它们在体内的反应调节有关。
Nonpeptidic compounds stimulate human T cells bearing the TCR-γδ in the absence of major histocompatibility complex restriction. We report that one of these ligands, 2,3-diphosphoglyceric acid (DPG), which induces expansion of Vγ9/Vδ T cells ex vivo, antagonizes the same cell population after repetitive activation. Stimulation with DPG results in partial early protein tyrosine phosphorylation and a prolonged, but reversible, state of unresponsiveness to agonist ligands in Vγ9/Vδ2, but not in other T cells. These findings show that TCR antagonism is a general phenomenon of T cells. However, in contrast to the clonal specificity of altered peptides antagonizing αβ T cells, all the tested Vγ9/Vδ2 polyclonal cell lines and clones become unresponsive, a fact that may be relevant for the regulation of their response in vivo.