DIAGNOSIS OF AIDS BY USING A 12-AMINO ACID PEPTIDE REPRESENTING AN IMMUNODOMINANT EPITOPE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS
DIAGNOSIS OF AIDS BY USING A 12-AMINO ACID PEPTIDE REPRESENTING AN IMMUNODOMINANT EPITOPE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS
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DOI：
10.1093/infdis/156.2.261
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发表时间：
1987-08-01
影响因子：
6.4
通讯作者：
OLDSTONE, MBA
中科院分区：
文献类型：
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作者：
GNANN, JW;SCHWIMMBECK, PL;OLDSTONE, MBA
我们绘制了人类免疫缺陷病毒（HIV）的免疫显性结构域。我们选择了由HIV的gag、pol和env基因的保守区编码的亲水性氨基酸序列作为潜在的抗原结构域。合成了代表这些序列的十八个肽；这些肽在兔子体内引起了强烈的抗体反应。对 53 名 HIV 感染者和 50 名对照者的血清进行了合成肽测试。尽管不存在针对来自 gag、pol 或 env gp120 蛋白的肽的抗体，但检测到针对来自 env gp41 的六种肽中的四种的抗体。使用重叠肽进行的表位作图显示，53 名 HIV 感染患者中的 53 名 (100%) 的血清（50 名对照者中没有一人）与 gp41 的肽 aa584-609 和 aa598-609 发生反应，53 名患者中的 32 名 (60%) 的血清与肽 aa603-614 发生反应，19 名患者 (35%) 的血清与肽 aa603-614 发生反应。 53 名患者与肽 aa609-620 发生反应。因此，跨膜糖蛋白的氨基酸序列LeuGlyIleTrpGlyCysSerGlyLysLeuIleCys (aa598-609)是HIV的免疫显性结构域，可被HIV感染者的血清抗体识别。
We mapped an immunodominant domain of the human immunodeficiency virus (HIV). We selected hydrophilic amino acid sequences encoded by conserved regions of the gag, pol, and env genes of HIV as potential antigenic domains. Eighteen peptides representing these sequences were synthesized; the peptides elicited strong antibody responses in rabbits. Sera from 53 HIV-infected patients and 50 controls were tested against the synthetic peptides. Although no antibodies to peptides from gag, pol, or env gp120 proteins were present, antibodies to four of the six peptides from env gp41 were detected. Epitope mapping using overlapping peptides showed that sera from 53 (100%) of 53 HIV-infected patients (and from none of 50 controls) reacted with peptides aa584-609 and aa598-609 from gp41, sera from 32 (60%) of 53 patients reacted with peptide aa603-614, and sera from 19 (35%) of 53 patients reacted with peptides aa609-620. Thus, amino acid sequence LeuGlyIleTrpGlyCysSerGlyLysLeuIleCys (aa598-609) from the transmembrane glycoprotein is an immunodominant domain of HIV recognized by serum antibodies from HIV-infected patients.