Protrudin induces neurite formation by directional membrane trafficking
Protrudin induces neurite formation by directional membrane trafficking
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DOI：
10.1126/science.1134027
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发表时间：
2006-11-03
期刊：
影响因子：
56.9
通讯作者：
Nakayama, Keiichi I.
中科院分区：
文献类型：
--
作者：
Shirane, Michiko;Nakayama, Keiichi I.
Rab家族的鸟苷三磷酸酶是膜运输的关键调节因子，其中Rab11在膜回收中发挥特定作用。我们发现了一种哺乳动物蛋白，突起蛋白，通过与鸟苷二磷酸（GDP）结合形式的Rab11相互作用促进神经突的形成。细胞外信号调节激酶（ERK）响应神经生长因子对突起蛋白的磷酸化促进了突起蛋白与Rab11-GDP的关联。RNA干扰下调突起蛋白可诱导膜向各方向延伸，抑制神经突的形成。因此，突起蛋白调节rab11依赖的膜再循环，促进神经突形成所需的定向膜运输。
Guanosine triphosphatases of the Rab family are key regulators of membrane trafficking, with Rab11 playing a specific role in membrane recycling. We identified a mammalian protein, protrudin, that promoted neurite formation through interaction with the guanosine diphosphate (GDP) - bound form of Rab11. Phosphorylation of protrudin by extracellular signal - regulated kinase (ERK) in response to nerve growth factor promoted protrudin association with Rab11-GDP. Down-regulation of protrudin by RNA interference induced membrane extension in all directions and inhibited neurite formation. Thus, protrudin regulates Rab11-dependent membrane recycling to promote the directional membrane trafficking required for neurite formation.