Genetically engineered protein in hydrogels tailors stimuli-responsive characteristics
Genetically engineered protein in hydrogels tailors stimuli-responsive characteristics
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DOI：
10.1038/nmat1352
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发表时间：
2005-04-01
期刊：
影响因子：
41.2
通讯作者：
Daunert, S
中科院分区：
文献类型：
--
作者：
Ehrick, JD;Deo, SK;Daunert, S
某些蛋白质在受到某种刺激后会发生明显的构象变化。这种构象变化可以以不同的方式表现出来，并导致驱动，即催化或信号事件、运动、与其他蛋白质的相互作用等，，，，，。在所有情况下，蛋白质的感应驱动过程都是由一个识别事件启动的，该事件转化为机械动作。因此，蛋白质是设计新型纳米材料的理想成分，这种材料是智能的，可以根据目标刺激执行所需的机械动作。已经采取了许多方法来模仿自然的感知驱动过程，，，，。我们现在报告了一种新的杂交材料，它将基因工程蛋白整合在能够产生刺激反应作用机制的水凝胶中。这种机械效应是由于蛋白质对刺激的反应引起的构象变化和结合亲和力。刺激反应水凝胶表现出三个特定的膨胀阶段，以响应各种配体，提供额外的微调控制，而不是传统的两阶段膨胀水凝胶。新制备的材料被用于生物分子在聚合物网络中的传感、随后的门控和运输，显示了其在微流体和小型化药物输送系统中的潜在应用。
Certain proteins undergo a substantial conformational change in response to a given stimulus. This conformational change can manifest in different manners and result in an actuation, that is, catalytic or signalling event, movement, interaction with other proteins, and so on,,,,,. In all cases, the sensing–actuation process of proteins is initiated by a recognition event that translates into a mechanical action. Thus, proteins are ideal components for designing new nanomaterials that are intelligent and can perform desired mechanical actions in response to target stimuli. A number of approaches have been undertaken to mimic nature's sensing–actuating process,,,,. We now report a new hybrid material that integrates genetically engineered proteins within hydrogels capable of producing a stimulus-responsive action mechanism. The mechanical effect is a result of an induced conformational change and binding affinities of the protein in response to a stimulus. The stimuli-responsive hydrogel exhibits three specific swelling stages in response to various ligands offering additional fine-tuned control over a conventional two-stage swelling hydrogel. The newly prepared material was used in the sensing, and subsequent gating and transport of biomolecules across a polymer network, demonstrating its potential application in microfluidics and miniaturized drug-delivery systems.