Release tags: a new class of analytical reagents.
Release tags: a new class of analytical reagents.
复制标题
发布标签：一类新型分析试剂。
DOI：
10.1093/clinchem/28.9.1844
复制
发表时间：
1982
影响因子：
9.3
通讯作者：
R. Giese
中科院分区：
文献类型：
--
作者：
R. Joppich;M. Joppich;R. Giese
释放标签是由三个分子组组成的新型化合物：“信号”、“释放”和“反应性”。“信号基团是标签的检测部分，释放基团提供了一个特定的共价切割位点，反应基团将标签连接到感兴趣的物质上。释放标签的一般目的是用作分子标记，类似于放射性同位素。释放标签还将气相色谱作为信号组的适用性扩展到非挥发性物质的分析。通过气相色谱-电子捕获检测器（GC-ECD）以高灵敏度和特异性测量这些化合物。由于GC-ECD分析仪可能具有广泛的多重性，因此许多类似但独特的释放标签可以同时用作给定测定中的标记物。在本文中，我们使用N-五氟苯甲酰甲硫氨酰甘氨酸-N-羟基琥珀酰亚胺酯，我们的第一个GC-ECD释放标签，测定血清中的甲状腺素的方法称为“标记分析”，其中涉及同位素衍生物分析的原理。
Release tags are novel compounds made up of three molecular groups: "signal," "release," and "reactivity." The signal group is the detected part of the tag, the release group provides a site for specific covalent cleavage, and the reactivity group attaches the tag to a substance of interest. The general purpose of release tags is to function as molecular labels, analogous to radioisotopes. Release tags also extend the applicability of gas-phase electrophores as signal groups to the analysis of nonvolatile substances. These electrophores are measured with high sensitivity and specificity by gas chromatography with electron-capture detection (GC-ECD). Because extensive multiplicity is potentially available with GC-ECD electrophores, many analogous but distinctive release tags can be used simultaneously as labels in a given assay. In this paper, we use N-pentafluorobenzoylmethionylglycine-N-hydroxysuccinimide ester, our first GC-ECD release tag, to determine thyroxin in serum by a method called "labeling analysis," which involves the principle of isotope-derivative analysis.