The effects of physiological adaptations to calorie restriction on global cell proliferation rates
The effects of physiological adaptations to calorie restriction on global cell proliferation rates
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DOI：
10.1152/ajpendo.00661.2010
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发表时间：
2011-04-01
影响因子：
5.1
通讯作者：
Hellerstein, Marc K.
中科院分区：
文献类型：
--
作者：
Bruss, Matthew D.;Thompson, Airlia C. S.;Hellerstein, Marc K.
1997年，李文亮.热量限制的生理适应对整体细胞增殖率的影响。Am J Physiol Endocrinol Metab 300：E735-E745，2011。首次出版于2011年2月1日; doi：10.1152/ajpendo. 00661.2010.-热量限制（CR）降低有丝分裂组织中细胞增殖的速率。已经表明，这种细胞增殖的减少可能介导CR诱导的寿命增加。然而，导致CR诱导的细胞增殖率降低的机制仍不清楚。为了评估CR诱导的生理适应，可能介导细胞增殖率的降低，我们改变了住房温度和获得自愿跑轮，以确定食物摄入量，能量消耗，体脂百分比和体重对角质形成细胞，肝细胞，乳腺上皮细胞和脾脏T细胞的增殖率的影响C57 BL/6小鼠。我们发现，与20% CR相似，所有细胞类型的细胞增殖率均降低。然而，与自由进食的对照组相比，在1）27 ℃下饲养的雌性小鼠的食物摄入和能量消耗减少，2）提供跑步轮的雌性小鼠的体脂百分比减少，或3）提供跑步轮的雄性小鼠的体重减少的情况下，没有观察到较低的细胞增殖率。相反，胰岛素样生长因子I的减少与细胞增殖率降低有关。总之，这些数据表明，CR诱导的食物摄入量，能量消耗，体脂百分比和体重的减少并不能解释CR中观察到的整体细胞增殖率的降低。此外，这些数据与假设一致，即细胞增殖率降低可以用作延长寿命的干预措施的生物标志物。
Bruss MD, Thompson AC, Aggarwal I, Khambatta CF, Hellerstein MK. The effects of physiological adaptations to calorie restriction on global cell proliferation rates. Am J Physiol Endocrinol Metab 300: E735-E745, 2011. First published February 1, 2011; doi: 10.1152/ajpendo. 00661.2010.-Calorie restriction (CR) reduces the rate of cell proliferation in mitotic tissues. It has been suggested that this reduction in cell proliferation may mediate CR-induced increases in longevity. However, the mechanisms that lead to CR-induced reductions in cell proliferation rates remain unclear. To evaluate the CR-induced physiological adaptations that may mediate reductions in cell proliferation rates, we altered housing temperature and access to voluntary running wheels to determine the effects of food intake, energy expenditure, percent body fat, and body weight on proliferation rates of keratinocytes, liver cells, mammary epithelial cells, and splenic T-cells in C57BL/6 mice. We found that similar to 20% CR led to a reduction in cell proliferation rates in all cell types. However, lower cell proliferation rates were not observed with reductions in 1) food intake and energy expenditure in female mice housed at 27 C, 2) percent body fat in female mice provided running wheels, or 3) body weight in male mice provided running wheels compared with ad libitum-fed controls. In contrast, reductions in insulin-like growth factor I were associated with decreased cell proliferation rates. Taken together, these data suggest that CR-induced reductions in food intake, energy expenditure, percent body fat, and body weight do not account for the reductions in global cell proliferation rates observed in CR. In addition, these data are consistent with the hypothesis that reduced cell proliferation rates could be useful as a biomarker of interventions that increase longevity.