Proteomic analysis provides new insight into the chicken eggshell cuticle
Proteomic analysis provides new insight into the chicken eggshell cuticle
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DOI：
10.1016/j.jprot.2012.03.019
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发表时间：
2012-05-17
影响因子：
3.3
通讯作者：
Hincke, Maxwell T.
中科院分区：
文献类型：
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作者：
Rose-Martel, Megan;Du, Jingwen;Hincke, Maxwell T.
角质层是鸟类蛋壳的最外层，其蛋白质成分几乎不为人所知。我们假设角质层成分在微生物耐药性中起主要作用，因为角质层不完整或缺失的鸡蛋更容易受到细菌污染。本研究从鸡蛋角质层最外层非钙化层提取蛋白质，并对其进行LC/MS/MS蛋白质组学分析。我们鉴定了47种角质层蛋白，具有高可信度和可重复性。两种蛋白，类似于kunitz样蛋白酶抑制剂和ovocalyxin-32（一种羧肽酶a抑制剂），是角质层蛋白中最丰富的蛋白。在卵子中检测到许多已知具有抗菌活性的蛋白（溶菌酶C、卵黄转铁蛋白、卵黄素-32、胱抑素、卵黄素抑制剂）以及可能的新候选蛋白（髓过氧化物酶、卵黄素-36和SERPIN家族成员）。这是首次全面报道角质层蛋白质组，这是确定角质层功能及其抗菌特性的分子基础的起点。(C) 2012 Elsevier B.V.版权所有
The cuticle is the outermost layer of the avian eggshell, whose protein constituents remain virtually unknown. We hypothesize that cuticle components play a major role in microbial resistance, since eggs with incomplete or absent cuticle are more susceptible to bacterial contamination. In this study we extracted proteins from the outermost non-calcified layer of the cuticle of chicken eggs and subjected them to LC/MS/MS proteomic analysis. We identified 47 cuticle proteins with high confidence and reproducibility. Two proteins, similar to Kunitz-like protease inhibitor and ovocalyxin-32 (a carboxypeptidase A inhibitor), were the most abundant of the cuticle proteins. A number of proteins known to have antimicrobial activity in the egg were detected (lysozyme C, ovotransferrin, ovocalyxin-32, cystatin, ovoinhibitor) as well as possible new candidates (myeloperoxidase, ovocalyxin-36 and members of the SERPIN family). This is the first comprehensive report of cuticle proteome, a starting point to determine cuticle function and the molecular basis of its antimicrobial properties. (C) 2012 Elsevier B.V. All rights reserved.