Mapping of immunoglobulin variable region genes: relationship to the 'deletion' model of immunoglobulin gene rearrangement.
Mapping of immunoglobulin variable region genes: relationship to the 'deletion' model of immunoglobulin gene rearrangement.
复制标题
免疫球蛋白可变区基因的定位：与免疫球蛋白基因重排的“缺失”模型的关系。
DOI：
10.1093/nar/9.21.5725
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发表时间：
1981
影响因子：
14.9
通讯作者：
Storb,U
中科院分区：
文献类型：
--
作者：
Selsing,E;Storb,U
采用Southern blot杂交技术对小鼠kappa链可变区基因的5个家族进行分析，确定其相对染色体图谱位置。通过从表达上游VKgene的抗体产生细胞中删除VKgene，并在表达下游基因的细胞中保留VKgene来推断图谱位置。在骨髓瘤M0PC167、MPC11、M0PC21和ABPC2O中表达的vk区分别是vkfamily的成员，分别表现出1、3、6和6个主要种系杂交带。5个家系的种系DNA基因序列为Vm167YM11 - (VM21)。VA20) - VABE8- JK- ck。正如在免疫球蛋白基因重排缺失模型中所预期的那样，在一些没有保留任何种系CKgenes的抗体产生细胞中，发现了位于种系DNA中j1仅5 '的序列缺失。然而，其他细胞系在重排环境中含有该序列，这表明任何免疫球蛋白V- j连接的缺失模型以及V基因定位都必须考虑“被删除”DNA的逐步重排和重新整合的可能性。
Five families of variable region genes of mouse kappa chains were analyzed by Southern blot hybridization to determine their relative chromosomal map positions. Map positions were deduced by VKgene deletion from anti-body-producing cells expressing upstream VKgenes and retention in cells expressing downstream genes. The VKregions expressed in the myelomas M0PC167, MPC11, M0PC21 and ABPC2O are members of VKfamilies exhibiting one, three, six and six major germline hybridization bands respectively. The gene order of the five families in germline DNA was found to be Vm167YM11 - (VM21. VA20) - VABE8- JK- ck. As expected in a deletion model of immunoglobulin gene rearrangement, a sequence located just 5′ of J1in germline DNA was found to be absent from some antibody producing cells which had not retained any germline CKgenes. However, other cell lines contained this sequence in rearranged contexts, suggesting that any deletion model of immunoglobulin V-J joining, as well as V gene mapping, must take into account the possibilities of stepwise rearrangements and reintegration of “deleted” DNA.