Functional characterization of Vip3Aa from Bacillus thuringiensis reveals the contributions of specific domains to its insecticidal activity.
Functional characterization of Vip3Aa from Bacillus thuringiensis reveals the contributions of specific domains to its insecticidal activity.
复制标题
苏云金芽孢杆菌 Vip3Aa 的功能表征揭示了特定结构域对其杀虫活性的贡献
DOI：
10.1016/j.jbc.2023.103000
复制
发表时间：
2023-03
影响因子：
4.8
通讯作者：
Gao, Xiang
中科院分区：
文献类型：
--
作者：
Jiang, Kun;Chen, Zhe;Zang, Yuanrong;Shi, Yiting;Shang, Chengbin;Jiao, Xuyao;Cai, Jun;Gao, Xiang
微生物衍生的、基于蛋白质的生物农药为合成农药提供了更可持续的害虫管理替代品。营养期杀虫蛋白（Vip 3）是苏云金芽孢杆菌分泌的多结构域蛋白，是第二代杀虫毒素，已初步应用于转基因作物。然而，Vip 3毒性的分子机制知之甚少。在这里，我们确定不同的功能和贡献的域的Vip 3Aa蛋白质对草地贪夜蛾幼虫的毒性。我们证明，Vip 3Aa域II和III（DII-DIII）结合中肠上皮，而DI是必不可少的Vip 3Aa的稳定性和毒性内富含蛋白酶的宿主昆虫中肠。DI-DIII可以被中肠蛋白酶激活，并表现出与全长Vip 3Aa相似的细胞毒性。此外，我们确定DV可以通过其聚糖结合活性结合围食基质，这有助于Vip 3Aa杀虫活性。综上所述，本研究提供了多方面的见解Vip 3Aa的作用模式，这将显着促进其杀虫机制的澄清和进一步的合理开发。
Microbially derived, protein-based biopesticides offer a more sustainable pest management alternative to synthetic pesticides. Vegetative insecticidal proteins (Vip3), multidomain proteins secreted by Bacillus thuringiensis, represent a second-generation insecticidal toxin that has been preliminarily used in transgenic crops. However, the molecular mechanism underlying Vip3’s toxicity is poorly understood. Here, we determine the distinct functions and contributions of the domains of the Vip3Aa protein to its toxicity against Spodoptera frugiperda larvae. We demonstrate that Vip3Aa domains II and III (DII-DIII) bind the midgut epithelium, while DI is essential for Vip3Aa’s stability and toxicity inside the protease-enriched host insect midgut. DI-DIII can be activated by midgut proteases and exhibits cytotoxicity similar to full-length Vip3Aa. In addition, we determine that DV can bind the peritrophic matrix via its glycan-binding activity, which contributes to Vip3Aa insecticidal activity. In summary, this study provides multiple insights into Vip3Aa’s mode-of-action which should significantly facilitate the clarification of its insecticidal mechanism and its further rational development.
登录
查看更多内容
影响因子：
4.6
作者：
Banyuls N;Hernández-Rodríguez CS;Van Rie J;Ferré J
通讯作者：
Ferré J
影响因子：
5.2
作者：
Hou X;Han L;An B;Cai J
通讯作者：
Cai J
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
4.2
作者：
Gomis-Cebolla, Joaquin;Ferreira dos Santos, Rafael;Ferre, Juan
通讯作者：
Ferre, Juan
影响因子：
4.8
作者：
Bae, Brian;Ohene-Adjei, Samuel;Nair, Satish K.
通讯作者：
Nair, Satish K.