Effects of estrogen on muscarinic acetylcholine receptors in the rat hippocampus
Effects of estrogen on muscarinic acetylcholine receptors in the rat hippocampus
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DOI：
10.1159/000084202
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发表时间：
2004-01-01
影响因子：
4.1
通讯作者：
Abdalla, FMF
中科院分区：
文献类型：
--
作者：
Cardoso, CC;Pereira, RTS;Abdalla, FMF
本研究的目的是调查是否不同的雌激素操作对成年雌性大鼠海马的毒蕈碱乙酰胆碱受体（mAChRs）的表达的影响。海马获自动情前期（对照）、卵巢切除2、10和15天、卵巢切除15天并用17 β-雌二醇处理7天以及卵巢切除后立即用17 β-雌二醇处理21天的大鼠。通过测定血浆雌二醇水平和子宫相对重量来监测大鼠的雌激素状态。[ 3 H]奎宁环基二苯甲酸酯（[H-3] QNB）结合研究表明，与对照大鼠相比，卵巢切除术时间依赖性地增加了海马中mAChR的数量。21天的雌二醇治疗避免了卵巢切除术的影响。然而，卵巢切除术15天后7天的雌二醇治疗轻微改变了mAChR的数量。总之，这些结果表明，卵巢切除术时间依赖性地增加大鼠海马中的mAChRs数量。此外，这些数据表明，在卵巢功能丧失后的特定时间段内开始雌二醇治疗可能有效地预防激素剥夺对海马的特定影响。
The aim of the present study was to investigate whether different estrogen manipulations have effects on the expression of muscarinic acetylcholine receptors ( mAChRs) in the adult female rat hippocampus. Hippocampus was obtained from rats in proestrus ( control), ovariectomized for 2, 10 and 15 days, ovariectomized for 15 days and treated with 17 beta-estradiol for 7 days, and treated with 17 beta-estradiol immediately after ovariectomy for 21 days. Rats' estrogen status was monitored by measuring estradiol plasma levels and uterus relative weight. [ 3 H] quinuclidinyl benzilate ([H-3] QNB) binding studies indicated that ovariectomy time-dependently increases the number of mAChRs in hippocampus when compared to those obtained from control rats. Estradiol treatments for 21 days avoid the effect of ovariectomy. However, the estradiol treatments for 7 days after 15 days of ovariectomy slightly change the number of mAChRs. In conclusion, these results showed that ovariectomy time-dependently increases mAChRs number in the rat hippocampus. In addition, these data suggest that treatment with estradiol initiated within a specific period of time after the loss of ovarian function may be effective at preventing specific effects of hormone deprivation on hippocampus.