Single particle ICP-MS method development for the determination of plant uptake and accumulation of CeO2 nanoparticles
Single particle ICP-MS method development for the determination of plant uptake and accumulation of CeO2 nanoparticles
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DOI：
10.1007/s00216-016-9565-1
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Shi, Honglan
中科院分区：
文献类型：
--
作者：
Dan, Yongbo;Ma, Xingmao;Shi, Honglan
二氧化铈纳米颗粒（CeO（2）NPs）是最广泛使用的工程纳米颗粒之一，将越来越多地释放到环境中。因此，了解它们在植物，特别是粮食作物中的吸收，运输和转化至关重要，因为它代表了人类消费的潜在途径。这项奋进面临的主要挑战之一是目前的分析方法不足以在环境相关浓度下表征和量化复杂生物样品中的纳米材料。本文采用单粒子电感耦合等离子体质谱（SP-ICP-MS）技术，对黄瓜、番茄、大豆和南瓜等4种植物地上部中的颗粒状铈（Ce）的粒径和粒径分布、颗粒浓度以及溶解态铈进行了同时检测。先前用于从番茄植物中提取金纳米颗粒的Macerozyme R-10酶的酶消化方法成功地适用于从所有四种植物中提取CeO 2NP。这项研究是第一个报告和证明存在溶解铈在植物幼苗芽暴露于CeO（2）纳米粒子水培。植物吸收和积累的程度似乎取决于植物种类，需要进一步系统研究其机制。
Cerium dioxide nanoparticles (CeO(2)NPs) are among the most broadly used engineered nanoparticles that will be increasingly released into the environment. Thus, understanding their uptake, transportation, and transformation in plants, especially food crops, is critical because it represents a potential pathway for human consumption. One of the primary challenges for the endeavor is the inadequacy of current analytical methodologies to characterize and quantify the nanomaterial in complex biological samples at environmentally relevant concentrations. Herein, a method was developed using single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) technology to simultaneously detect the size and size distribution of particulate Ce, particle concentration, and dissolved cerium in the shoots of four plant species including cucumber, tomato, soybean, and pumpkin. An enzymatic digestion method with Macerozyme R-10 enzyme previously used for gold nanoparticle extraction from the tomato plant was adapted successfully for CeO2NP extraction from all four plant species. This study is the first to report and demonstrate the presence of dissolved cerium in plant seedling shoots exposed to CeO(2)NPs hydroponically. The extent of plant uptake and accumulation appears to be dependent on the plant species, requiring further systematic investigation of the mechanisms.