Controlling the membrane fluidity of yeasts during coupled thermal and osmotic treatments
Controlling the membrane fluidity of yeasts during coupled thermal and osmotic treatments
复制标题
在热和渗透联合处理过程中控制酵母的膜流动性
DOI：
10.1002/bit.21749
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发表时间：
2008
影响因子：
3.8
通讯作者：
P. Gervais
中科院分区：
文献类型：
--
作者：
H. Simonin;L. Beney;P. Gervais
酵母在发酵工业中使用时，经常暴露于其天然环境或其底物中的渗透压变化。这种变化可能导致细胞死亡或活性丧失。我们团队以前的工作使我们能够将暴露于热和渗透处理组合的细胞的死亡率与脂质相变发生时细胞组分通过不稳定膜的泄漏联系起来。在这项研究中，酵母活力测定后，许多渗透和热处理。此外，根据aw和温度，通过1，6-二苯基-1，3，5-己三烯（DPH）各向异性测量来评估酵母膜的流动性。结果表明，有一个负相关性的整体流动性的变化，在处理过程中所经历的膜和酵母存活。使用图膜流动性根据aw和温度，我们定义了脱水和再水化的方法，最大限度地减少流动性波动，允许显着增加酵母菌的生存。因此，这样的膜流动性图应该是一个潜在的工具，用于控制膜状态在脱水和复水和提高酵母存活。整体流动性测量现在应该通过准确的膜结构分析来完成，以更好地了解脱水和再水化过程中发生的质膜变化。Biotechnol. Bioeng. 2008;100：325-333.© 2007 Wiley Periodicals，Inc.
Yeasts are often exposed to variations in osmotic pressure in their natural environments or in their substrates when used in fermentation industries. Such changes may lead to cell death or activity loss. Previous work by our team has allowed us to relate the mortality of cells exposed to a combination of thermal and osmotic treatments to leakage of cellular components through an unstable membrane when lipid phase transition occurs. In this study, yeast viability was measured after numerous osmotic and thermal treatments. In addition, the fluidity of yeast membranes was assessed according to aw and temperature by means of 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) anisotropy measurement. The results show that there is a negative correlation between the overall fluidity variation undergone by membranes during treatments and yeast survival. Using a diagram of membrane fluidity according to aw and temperature, we defined dehydration and rehydration methods that minimize fluidity fluctuations, permitting significantly increased yeast survival. Thus, such membrane fluidity diagram should be a potential tool for controlling membrane state during dehydration and rehydration and improve yeast survival. Overall fluidity measurements should now be completed by accurate structural analysis of membranes to better understand the plasma membrane changes occurring during dehydration and rehydration. Biotechnol. Bioeng. 2008;100: 325–333. © 2007 Wiley Periodicals, Inc.
影响因子：
3.4
作者：
ARAKAWA, T;TIMASHEFF, SN
通讯作者：
TIMASHEFF, SN
影响因子：
3.4
作者：
Binder, H;Gawrisch, K
通讯作者：
Gawrisch, K