Culturable bacterial community analysis in the root domains of two varieties of tree peony (Paeonia ostii)
Culturable bacterial community analysis in the root domains of two varieties of tree peony (Paeonia ostii)
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两个牡丹品种根域可培养细菌群落分析
DOI：
10.1111/j.1574-6968.2011.02319.x
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Hu, Yonghong
中科院分区：
文献类型：
--
作者：
Han, Jigang;Song, Yao;Hu, Yonghong
从牡丹品种“凤丹”和“兰芙蓉”的根部分离到985株具有不同菌落特征的细菌，经扩增核糖体DNA酶切分析鉴定出69个操作分类单位。选择各组代表进行部分16 S rRNA基因测序和系统发育分析。丰丹土壤、根际和根面中的主要类群分别为厚壁菌门（63.2%）、放线菌门（36.3%）和β-变形菌门（53.0%）。兰芙蓉根际土壤、根际土壤和根面土壤中的主要细菌类群分别为放线菌（34.8%）、γ-变形菌（45.2%）和β-变形菌（49.1%）。总的来说，细菌分离物包括26个属- 14个在散装土壤中，14个在根际，11个在根面。丰丹和兰芙蓉的根际土壤中以芽孢杆菌属（49.6%和32.6%）、微杆菌属（21.1%）和假单胞菌属（42.0%）、根际Variovorax属（53.0%和49.1%）最常见。结果表明，两个品种3个根域的细菌群落结构存在明显差异，植物对其相关细菌种群产生选择压力。寄主基因型也影响细菌群落的分布格局。
A total of 985 bacterial strains with different colony characteristics were isolated from the root of tree peony plants (variety 'Fengdan' and 'Lan Furong'); 69 operational taxonomic units were identified by amplified ribosomal DNA restriction analysis. Representatives of each group were selected for partial 16S rRNA gene sequencing and phylogenetic analysis. The major groups in the bulk soil, rhizosphere, and rhizoplane of Fengdan were Firmicutes (63.2%), Actinobacteria (36.3%), and Betaproteobacteria (53.0%), respectively. The major bacteria groups in the bulk soil, rhizosphere, and rhizoplane of Lan Furong were Actinobacteria (34.8%), Gammaproteobacteria (45.2%), and Betaproteobacteria (49.1%), respectively. In total, the bacterial isolates comprised 26 genera - 14 in the bulk soil, 14 in the rhizosphere, and 11 in the rhizoplane. The most common genus in the bulk soil of Fengdan and Lan Furong was Bacillus (49.6% and 32.6%, respectively), in the rhizosphere Microbacterium (21.1%) and Pseudomonas (42.0%), and in the rhizoplane Variovorax (53.0% and 49.1%, respectively). The results show that there are obvious differences in the bacterial communities in the three root domains of the two varieties, and the plants exerted selective pressures on their associated bacterial populations. The host genotypes also influenced the distribution pattern of the bacterial community.