Detection of malignant pleural mesothelioma in exhaled breath by multicapillary column/ion mobility spectrometry (MCC/IMS)
Detection of malignant pleural mesothelioma in exhaled breath by multicapillary column/ion mobility spectrometry (MCC/IMS)
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DOI：
10.1088/1752-7155/10/4/046001
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发表时间：
2016-12-01
影响因子：
3.8
通讯作者：
van Meerbeeck, Jan P.
中科院分区：
文献类型：
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作者：
Lamote, Kevin;Vynck, Matthijs;van Meerbeeck, Jan P.
恶性胸膜间皮瘤（MPM）主要是由以前接触石棉引起的。诊断通常发生在晚期，限制了任何治疗前景。利用多毛细管柱/离子迁移谱法（MCC/IMS）对MPM患者呼出气体中的挥发性有机化合物（VOCs）进行早期检测，并与前职业石棉暴露和非暴露对照进行比较。对23例MPM患者、22例无症状前石棉（AE(x)）工人和21例健康无石棉暴露者的呼吸和背景样本进行分析。在进行背景校正后，我们进行了逻辑最小绝对收缩和选择算子（lasso）回归来选择最重要的VOCs，然后进行受试者工作特征（ROC）分析。将MPM患者与对照组区分开来的敏感性为87%，特异性为70%，阳性和阴性预测值分别为61%和91%。总体准确度为76%，roc曲线下面积为0.81。AEx个体与MPM患者区分的敏感性为87%，特异性为86%，阳性预测值为87%，阴性预测值为86%。总体准确度为87%，roc曲线下面积为0.86。MCC/IMS呼气分析可以将MPM患者与对照组区分开来，并有望作为前石棉暴露者患MPM风险的筛查工具进行进一步调查。
Malignant pleural mesothelioma (MPM) is predominantly caused by previous asbestos exposure. Diagnosis often happens in advanced stages restricting any therapeutic perspectives. Early stage detection via breath analysis was explored using multicapillary column/ion mobility spectrometry (MCC/IMS) to detect volatile organic compounds (VOCs) in the exhaled breath of MPM patients in comparison to former occupational asbestos-exposed and non-exposed controls.Breath and background samples of 23 MPM patients, 22 asymptomatic former asbestos (AE(x)) workers and 21 healthy non-asbestos exposed persons were taken for analysis. After background correction, we performed a logistic least absolute shrinkage and selection operator (lasso) regression to select the most important VOCs, followed by receiver operating characteristic (ROC) analysis.MPM patients were discriminated from both controls with 87% sensitivity, 70% specificity and respective positive and negative predictive values of 61% and 91%. The overall accuracy was 76% and the area under the ROC-curve was 0.81. AEx individuals could be discriminated from MPM patients with 87% sensitivity, 86% specificity and respective positive and negative predictive values of 87% and 86%. The overall accuracy was 87% with an area under the ROC-curve of 0.86.Breath analysis by MCC/IMS allows MPM patients to be discriminated from controls and holds promise for further investigation as a screening tool for former asbestos-exposed persons at risk of developing MPM.